1996 Fiscal Year Final Research Report Summary
Nien9C oxiok in vasospasm folloroing experimenibl subamchnoid hemo rrhege
| Project/Area Number |
07671495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Tohoku University |
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Principal Investigator |
TOMINAGA Teiji Research Associate school of Medicine hospit Dept.Neuresurgory, Tohoku Univ, 医学部・附属病院, 助手 (00217548)
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| Project Period (FY) |
1995 – 1996
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| Keywords | subarachnoid hemorrhage / vasospasm / free radical / SOD / mouse |
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| Research Abstract |
The purpose of the present study is to examine whether superoxide dismutase (SOD) suppresses experimental vasospasm following subarachnoid hemorrhage (SAH) using transgenic mice overexpressing human Cu-Zu SOD.This transgenic mouse was produced by insertion of human CuZn-SOD gene to the MCH (IRC) mouse, and has three times of SOD activity compared with non-transgenic mouse. The experimental SAH was produced by perforation of the anterior cerebral artery with a nylon suture inserted from the internal carotid artery. The mice were perfused from the ascending aorta with 10% formalin solution followed by carbon gel solution 4 hours, 1 day, 3 days, 7 days, and 14 days after the SAH.The diameter of the proximal middle cerebral artery (MCA) was measured under phase-contrast microscope. The survival rate of the SOD-transgenic mice 3 days after SAH,approximately 70%, was not statistically different from that of non-transgenic mice. The MCA diameter of non-transgenic mice without SAH was 138.5(]SY.+-。[)14.5mum(control value, mean(]SY.+-。[)SD,n=12). The MCA diameter of non-transgenic mice did not change compared with the control values 4 hours, 1 day, 7 days, and 14 days after the SAH,and significantly decreased to 110.5(]SY.+-。[)20.5mum (n=16, p<0.01) only 3 days after the SAH.Three days after the SAH,the MCA diameter of the SOD-transgenic mice was larger than that of the non-transgenic mice with statistical significance (127.9(]SY.+-。[)20.2mum, n=20 ; p<0.05). These results suggest that CuZn-SOD can mitigate vasospasm caused by SAH and that the mechanism underlying vasospasm includes free radical reactions associated with superoxide anion.
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