1996 Fiscal Year Final Research Report Summary
Study of factors that regulates site-specific brain metastasis
| Project/Area Number |
07671500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
FUJIMAKI Takamitsu The University of Tokyo, Department of Neurosurgery, Faculty of Medicine, Associate, 医学部・附属病院, 助手 (80251255)
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| Co-Investigator(Kenkyū-buntansha) |
IRIMURA Tatsuro The University of Tokyo, Department of Chemical Toxicity and Immunology, Faculty, 薬学部, 教授 (80092146)
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| Project Period (FY) |
1995 – 1996
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| Keywords | melanoma / brain metastasis / site-specific brain metastasis / TGF-beta |
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| Research Abstract |
Mouse B16 melanoma cells which originally does not grow in the brain parenchyma were selected by modified Boyden chamber method for adhesiveness to mouse brain endotherial cells. Intracarotid injection of these cell produced intraparenchymal growth in some mice. However the difference in the pattern of growth of these cells from that of parent cell line was not significant, i. e. the adhesiveness to brain endotherial cells is not a major factor to determine site specific metastasis to brain parenchyma.
Six human melanoma cell lines derived from cutaneous lymph node or brain metastases and melanoma cells isolated from fresh surgical specimens of two primary cutaneous melanomas, two lymph node metastases and two brain metastases (each from a different patient) were injected into the subarachnoid space of nude mice. All melanomas produced growths in the leptomeninges, but only melanoma cells isolated from brain metastases infiltrated into and grew in the brain parenchyma of nude mice. The growth in vitro of human melanoma cells in the presence of TGF-beta inversely correlated with potential for brain parenchyma metastasis. These data suggest that infiltrative growth of melanoma cells into the brain parenchyma after intracistemal injection is a good model to assess the capacity of each melanoma to metastasize to brain parenchyma. These data also suggest that melanoma brain parenchymal metastases are produced by unique cells that may be resistant to the antiproliferative effects of TGF-beta.
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