1996 Fiscal Year Final Research Report Summary
The role of cytokines after focal cerebral ischemia
| Project/Area Number |
07671542
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Teikyo Univ.School of Med. |
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Principal Investigator |
NAKANE Makoto Teikyo Univ.School of Med., Dept.of Neurosurgery, Assistant, 医学部, 助手 (00266305)
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| Co-Investigator(Kenkyū-buntansha) |
KANEMITSU Hitdeaki Teikyo Univ.School of Med., Dept.of Neurosurgery, Assistant Prof., 医学部, 講師 (10129992)
NAKAGOMI Tadayoshi Teikyo Univ.School of Med., Dept.of Neurosurgery, Accociate Prof., 医学部, 助教授 (90198052)
TAMURA Akira Teikyo Univ.School of Med., Dept.of Neurosurgery, Professor, 医学部, 教授 (80111532)
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| Project Period (FY) |
1995 – 1996
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| Keywords | cytokine / mouse / rat / MCA occlusion / cortical ablation / MRI |
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| Research Abstract |
To detect secondary changes which occur either in the thalamus or the substantia nigra following middle cerebral artery (MCA) occlusion in rats by means of magnetic resonance (MR) imaging. These secondary changes were observed in clinical cases after cortical and/or striatal infarction. Sprague-Dawley rats were subjected to MCA occlusion. At varying intervals, proton density-(PD-), T1-and T2-weighted imaging were performed. A 4.7-Tesla superconductive magnetic resonance imager was used. T2-weighted images (T2WI) revealed the presence of a high signal intensity area in the ipsilateral substantia nigra 4 days after occlusion. A low signal intensity lesion in tne ipsilateral thalamus appeared 7 days after surgery as revealed by PDWI and/or T2WI.This is the first report of detection of secondary changes in the thalamus and the substantia nigra following MCA occlusion in rats using MR.MR should proved more information on the neuhropathology of the delayd neuronal degeneration after cerebral ischemia.
Immunohistochemical studies showed that IL-6 were induced in the ipsilateral internal capsule and cerebral peduncle 7 days after MCA occlusion in the mouse, however, IL-6 was not detected in the ipsilateral thalamus and substantia nigra where secondary neuronal degeneration occured gradually.
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