1996 Fiscal Year Final Research Report Summary
Basic study of discogenic low back pain.
| Project/Area Number |
07671570
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Chiba University |
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Principal Investigator |
TAKAHASHI Kazuhisa Department of Orthopaedic Surgery, School of Medicine Chiba University, Lecturer, 医学部, 講師 (20179477)
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| Project Period (FY) |
1995 – 1996
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| Keywords | low back pain / intervertebral disc / lumbar facet joint / innervation |
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| Research Abstract |
Forty-five Wistar rats were used. Seven days after the resection of sympathetic trunks with ganglia at different levels, the whole lumbar spine was stained by acetylcholinesterase histochemical method. The dense nerve network on the posterior portion of lumbar intervertebral discs had disappeared almost completely after total resection of bilateral sympathetic trunks at L2-L6. However, there was slight decrease in the network after bilateral single-level resection or unilateral multisegmental resection. The results showed the posterior portion of lumbar intervertebral discs was innervated by the sympathetic nerves multisegmentally and bilaterally.
To determine the sensory pathway, Cholera toxin B subunit, a neural tracer, was placed in the L5-L6 facet joint, and the bilateral dorsal root ganglia and paravertebral sympathetic ganglia were examined immunohistochemically. The serial sections of lumbar vertebrae of newborn rats and the sections of the facet joint capsules, dorsal root ganglia, and paravertebralsympathetic ganglia of adult rats were investigated immunohistochemically. Labeled neurons existed in ipstilateral dorsal root ganglia from L1 to L6 and in paravertebral sympathetic ganglia from T12 to L6. The dorsal ramus of the spinal nerve and rami communicantes were connected to each other by calcitonin gene-related peptide immunoreactive fibers and dopaminebeta-hydroxylase immunoreactive fibers.
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