1996 Fiscal Year Final Research Report Summary
A STUDY ON ANTI-INFLAMMATORY ACTION OF A NEW ENDOGENOUS OPIOIDE-REGULATED SUBSTANCE
| Project/Area Number |
07671687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | JUNTENDO UNIVERSITY |
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Principal Investigator |
KUGIMIYA Toyoki JUNTENDO UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (90010537)
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| Co-Investigator(Kenkyū-buntansha) |
HAZATO Tadahiko THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE,PRINCIPAL SCIENTIST, 主任研究員 (60109949)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Spinorphin / Carrageenan / Aminopeptidase N / Leuhistin / Anti-inflammatory |
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| Research Abstract |
Spinorphin is an endogenous pain-regulated substance which modulated an enkephalin-degrading activity. In this study, we found that Spinorphin causes a significant inhibition of inflammatory responses by human PMNs stimulated with FMLP and may be a new endogenous anti-inflammatory peptide which is mediated in part through the suppression in [Ca^<2+>]i changes. Next we examined the effects of Spinorphin, using an in vivo system involving mouse air-pouch as a model of acute inflammatory response. In this model carrageenan and PMNs accumulation in inflammatory sites were used as a inflammation-tiggerring agent and PMNs accumulation at inflammatory sites was an indicator of an acute inflammatory response. Intravenous administration of Spinorphin within air-pouch site elicited by carrageenan, the inhibition being 64%, compared to the saline-treated group. Combination of Leuhistin, and inhibitor of aminopeptidase N,sinergestically suppressed PMNs accumulation induced carrageenan. In the future, Spinorphin will be an important tool for use in clarifying of neuropeptide-induced biological phenomena such as enkephalin mediated pain and inflammation.
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