• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1997 Fiscal Year Final Research Report Summary

Tumor Cell Invasion or Metastasis Related to Calcium Sensitivity of Intracellular Locomotive Apparatus in Bladder Cancer

Research Project

Project/Area Number 07671743
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionFukushima Medical University, School of Medicine

Principal Investigator

YAMAGUCHI Osamu  Fukushima Medical University, School of Medicine Dept.of Urology, Professor, 医学部・泌尿器科, 教授 (60006814)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Yasukuni  Fukushima Medical University, School of Medicine Dept.of Urology, Lecturer, 医学部・泌尿器科, 講師 (50220744)
YOKOTA Takashi  Fukushima Medical University, School of Medicine Dept.of Urology, Lecturer, 医学部・泌尿器科, 講師 (20240939)
Project Period (FY) 1995 – 1997
Keywordsbladder tumor / bradykinin / calcium ion / contractile protein / cell metility / invasion / metastasis
Research Abstract

Tumor Cell Invasion or Metastasis Related to Calcium Sensitivity of Intracellular
Locmotive Apparatus in Bladder Cancer
At first, using mouse bladder tumor cells (MBT-2 cells), the present study evaluated the functional characteristics of the actomyosin system in bladder cancer cells. The results suggest that MBT-2 cells possess a locomotive apparatus consisting of actin and myosin, and that Ca^<2+> can activate this actomyosin system, leading to the contraction or active locomotory movement of tumor cells. When cancer cells actually begin to move in the in vivo situation, it is assumed that the cells need some biological substances which can elevate the cytoplasmic Ca^<2+> level.
As a candidate of such biological substances, we studied the effect of bradykinin (BK). BK was demonstrated to induce the transient rise of cytoplasmic concentration of Ca^<2+> in MBT-2 cells. Simultaneously with the increase in Ca^<2+> level, MBT-2 cells showed contraction. BK-induced Ca^<2+> transients were blocked by B2 inhibitor, but not by B_1 inhibitor, suggesting that this action of BK is mediated by B_2 subtypes.
When MBT-2 cells were incubated with BK, the number of cells migrated through matrigel-coated filter was significantly greater than the control without BK.Thus, BK was also shown to induce chemoinvasion.
RT-PCR clearly showed B_1 and B_2 receptor mRNAs were expressed in human bladder cancer and mouse bladder cancer. The amounts of B_2 mRNAs were 10 times greater than that of B_1 mRNAs. In addition, mRNAs of plasminogen activator, which cause a BK generation, were detected in human bladder cancer.
Thus, the results from this study suggest that BK may induce locomotory movement of bladder cancer cells by mobilizing Ca^<2+> and activating actomyosin system (locomotory apparatus), which result in tumor cell invasion and metastasis.

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi