1997 Fiscal Year Final Research Report Summary
The animal study of fetal therapy for genetic disease by names of fetal cells transplantation
| Project/Area Number |
07671762
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
UEHARA Shigeki Tohoku University Hospital, Lecturer, 医学部付属秒便, 講師 (10168651)
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| Project Period (FY) |
1995 – 1997
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| Keywords | fetal liver hematopoietic cell / in utero transplantation / intraperitoneal transplantation / colony assay / cell differentiation |
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| Research Abstract |
To investigate the possibility of engrafting fetal liver hematopoietic cells by in utero intraperitoneal transplantation, we transplanted donor cells obtained from mouse fetuses at 13,15 and 17 days of gestation to mouse fetuses at 15,16 and 17 days gestation. Engraftment was assessed by Sry gene amplification of DNA extracted from peripheral blood samples of transplanted mice sex weeks after birth. In comparison, we performed an in vitro colony-assay of fetal liver cells at 13,15 and 17 days of gestation. The incidence of engraftment was significantly higher in cells of 15 days of gestation than in cells of 13 or 17 days of gesation, whereas the colony forming activity decreased gradually from 13 to 15 days of gestation. From these results, we suggest that the 15 day liver contains hematopoietic progenitors which have the specific characteristics required for engraftment by intraperitoneal transplantation.
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