1997 Fiscal Year Final Research Report Summary
Immunological role of HLA-G for maintaining pregnancy
| Project/Area Number |
07671768
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Tokyo |
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Principal Investigator |
FUJII Tomoyuki University of Tokyo, Hospital, Assistant Professor, 医学部・附属病院, 講師 (40209010)
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| Project Period (FY) |
1995 – 1997
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| Keywords | HLA-G / pregnancy / Immunology / preeclampsia / cytokine / Natural Killer Receptor / Trophoblast |
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| Research Abstract |
1. Polymorphism of HLA-G.
We discovered a new allele of HLA-G which possessed a non-synonymous singlebase substitution in exon 3 compared with the previously reported alleles and was officially designated G**0104 by the WHO Nomenclature Committee. G**0104 was most common in Japanese population. In addition, we identified a single-base error in exon 2 in the sequence of G**01012 and corrected it.
2. HLA-G and preeclampsia.
The expression of HLA-G protein on the extravillous trophoblasts in placenta was attenuated in all the preeclamptic patients. All of these trophoblasts possessd IL-2 which was not observed in normal placenta. Further, serum concentrations of IL-2 in the first trimester of pregnancy in women who had preeclampsia develop after 28 weeks of pregnancy were higher than those of normal pregnant women. IL-2 in preeclamptic placenta might reduce the angiogenic substances arising from trophoblasts by inducing lymphokine activated killer cells from decidual lymphocytes, which might be relevant to deranged vasculature of the placenta, a characteristic histology in preeclampsia.
3. Cytokine release from mononuclear cells and HLA-G on the target cells.
Mononuclear cells, if cultured with HLA-G-expressing cells, increase their ability to release IL-3 which enhances the growth and development of trophoblasts and decrease TNF-alpha which suppresses the growth of trophoblasts.
4. Natural killer receptor on the decidual lymphocytes.
Decidual lymphocytes express the natural killer receptors which recognize HLA-G on the trophoblasts.
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[Publications] Miki, A, Ishikawa, Y, Yabe, T, Furukawa, H, Fujii, T, Tokunaga, K, et al: "Human lymphocytes obtained from decidual tissue express killer activatory receptors(KARs)as well as killer inhibitory receptors(KIRs)Analysis using a single strand conformation polymorphism(SSCP)method." Am J reprod Immuuol. (in press). (1998)
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