1996 Fiscal Year Final Research Report Summary
Study on the mechanism of lymphatic metastasis of uterine cancer and its inhibition
| Project/Area Number |
07671798
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KAMURA Toshiharu Faculty of Medicine, Kyushu University Associate professor, 医学部, 助教授 (30152870)
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| Co-Investigator(Kenkyū-buntansha) |
UEHIRA Kenji Faculty of Medicine, Kyushu University Lecturer, 医学部, 助手 (60274453)
KAKU Tunehisa Faculty of Medicine, Kyushu University Assistant professor, 医学部, 講師 (60185717)
KOBAYASHI Hiroaki Faculty of Medicine, Kyushu University Lecturer, 医学部, 助手 (70260700)
SAITO Toshiaki Faculty of Medicine, Kyushu University Assistant professor, 医学部, 講師 (80162212)
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| Project Period (FY) |
1995 – 1996
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| Keywords | UTERINE CANCER / LYMPH NODE METASTASIS / PROGNOSTIC FACTOR / LYMPH NODE CELLS / ANTIPLOLIFERATIVE ACTIVITY / PHYTOHEMAGGLUTININ / TUMOR NECROSIS FACTOR alpha |
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| Research Abstract |
The aims of this research were to investigate (1) if lymphatic metastasis influenced on survival significantly in the uterine cancer patients, and (2) if regional lymphatic cells had inhibitory potential against metastasis.
In uterine cervical cancer, pelvic lymph node metastasis had a strongest influence on survial among histopathological prognostic factors. Moreover, metastases in 2 or more pelvic lymph node gave worse survival than those in none or one lymph node. In endometrial cancer, paraaortic lymph node metastasis was seen in 9% of all patients. Paraaortic node positive patients had 5-year survival of 38% while paraaortic node negative patients. From these results, it was clear that regional lymph node metastasis significant prognostic factor.
Regional Lymph node cell obtained from uterine cancer patients exerted a significant antiproliferative activity against an endometrial cancer cell line RL95-2 on a human tumor clonogenic assay when stimulated with phytohemagglutinin (PHA). The PHA stimulated lymph node cells produced and released a high amount of tumor necrosis factor alpha (TNF alpha) and interferons. The antiproliferative activity was thought to be partly due to released TNF alpha, because RL95-2 was highly sensitive to recombinant TNF alpha. However anti-TNF alpha failed to inhibit the activity, which indicated that the probability of some as yet unclarified participation of cytokines. Therefore, the regional lymph nodes might be used as sites of endogeneous cytokine therapy.
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