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1996 Fiscal Year Final Research Report Summary

Relationship between pregnant uterine smooth muscle contraction and clinical disease

Research Project

Project/Area Number 07671836
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionFukuoka University

Principal Investigator

IZUMI Hidetaka  School of Medicine, Fukuoka University, Lecturer, 医学部・産婦人科, 講師 (10193377)

Co-Investigator(Kenkyū-buntansha) MAKINO Yasuo  School of Medicine, Fukuoka University, Assistant Professor, 医学部・産婦人科, 助手 (90248500)
Project Period (FY) 1995 – 1996
Keywordsmyometrium / uterine contraction / calcium / G-protein / oxytocin / nitric oxide / gestation
Research Abstract

In pregnant uterine smooth muscle cells, calcium (Ca^<2+>) plays an essential role in triggering contraction, as obserbed in other visceral smooth muscles. An increase of intracellular Ca^<2+> ([Ca^<2+>]i") in myometrial cells forms Ca^<2+>-calmodulin complex and phosphorylate myosin light chain and thus initiates myometrial cell contractions. To study the mechanism of contraction regulated by Ca^<2+>, chemically skinned muscle technique which permits membrane permeabilization and "chemical clamping of [Ca^<2+>]i" has been developed in smooth muscles of rat or human myometrium. Recently, it has been reported that agonist coupling to receptors increases Ca^<2+> sencitivity for contractile protein of vascular smooth muscles through activation of unidentified G-proteins. In pregnant rat myometrium, the maximum amplitude of tension induced by carbachol was much greater than that by KC1 in intact strips using calcium indicator while the maximum increase of [Ca^<2+>]i were alsmost same. In b … More eta-escin treated skinned myometrial strips from late gestation, 0.3 muM Ca^<2+> evoked contractions. Carbachol plus GTP or oxytocin plus GTP enhanced the 0.3 muM Ca^<2+>-induced contractions of skinned strips and the increase was antagonized by GDPbetaS.GTPgammaS,PGF2alpha plus GTP,PGE2 plus GTP,okadaic acid and protein kinase C (PKC) stimulated by PDBu also augmented 0.3 muM Ca^<2+> contractions in skinned strips. These results suggest that agonists, such as corbachol, oxytocin, PGs enhanced the Ca^<2+>-induced contraction of myometrium through G-protein mediated mechanism. These agonists/G-protein mediated Ca^<2+>-sensitizing effects on contractile elements might produces the additional contractile force with same amount of [Ca^<2+>]i this providing expelling forces for delivering of fetuses. In addition, nitric oxide, which stiimulates guanylate cyclase in the muscle cells to produce cGMP,inhibit pharmaco-mechanical coupling, contractile apparatus and Ca^<2+> extrusion system in pregnant rat myometrium. We believe that clarification of these intracellular phenomenon is usefull for the prevention of premature labor. Less

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] RE Garfield,Izumi H.: "Role of gap junction and nitric oxide in control of myometrial contractility." Semin Perinatol. 19. 41-51 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H,Garfield RE.: "Relaxant effects of nitric oxide and cyclic GMP on pregnant rat uterine longitudinal smooth muscle." Euro J Obstet Gynecol and repro Biol. 60. 171-180 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H,Smith MB,et al.: "Gestational changes in oxytocin and endothelin-1 induced contractility of pregnant rat myometrium." Euro J Pharmacol. 278. 187-194 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H,Makino Y,et.al.: "Role of nitric oxide on vasorelaxation in human umbilicalartery." Am J Obstet Gynecol. 172. 1477-1488 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H,Bian K,et.al.: "Agonists increase the sensitivity of contractile elements for Ca in pregnant rat myometrium." Am J Obstet Gynecol. 175. 199-206 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H,Makino Y et.al.: "Comparison of nitric oxide and prostacyclin in endothelium-dependent vasorelaxation of human umbilical artery at midgestation." Am J Obstet Gynecol. 175. 375-381 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H.: "Ca2+modulation system of myometrial contraction during gestation." Garfield RE and Tabb TN eds. Control of Uterine Contractility. Boca Raton,Ann Arbor,London,Tokyo : CRC Press, 441 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Izumi H,Garfield RE.: "Effects of oxytocin and guanosine nucleotides on skinned muscle tissues of pregnant rat myometrium." J.Soc Gynecol Invest. 2 (2) (Suppl). 262 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] RE Garfield, Ali M,Yallampalli C,Izumi H.: "Role of gap junction and nitric oxide in control of myometrial contractility." Semin Perinatol. 19. 41-51 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H,Garfield RE.: "Relaxant effects of nitric oxide and cyclic GMP on pregnant rat uterine longitudinal smooth muscle." Euro J Obstet Gynecol and Repro Biol. 60. 171-180 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H,Smith MB,Garfield RE.: "Gestational changes in oxytocin and endothelin-1 induced contractility of pregnant myometrium." Euro J Pharmacol.278. 187-194 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H,Makino Y,Shirakawa K,Garfield RE.: "Role of nitric oxide on vasorelaxation in human umbilicalartery." Am J Obstet Gynecol. 172. 1477-1488 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makino Y,Izumi H,Shirakawa K,Garfield RE.: "The effect of nitric oxide on in-vitro contractility and flow velocity wave form of umbilical artery in preeclampsia." J.Soc. Gynecol. Invest.(Abstract P) 309A.(1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H,Emoto M,Bukoski RD,Bian K,Garfield RE.: "Agonist increase the sensitivity of contractile elements for Ca in the pregnant rat myometrium." J.Soc. Gynecol. Invest.(Abstract P) 344A. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H,Bian K,Bukoski RD,Garfield RE.: "Agonists increase the sensitivity of contractile elements for Ca in the pregnant rat myometrium." Am J Obstet Gynecol. 175. 199-206 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H,Makino Y,Motai H,Shirakawa K,Garfield RE.: "Comparison of nitric oxide and prostacyclin in endothelium-dependent vasorelaxation of human umbilical artery at midgestation" Am J Obstet Gynecol. 175. 375-81 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Izumi H.: Ca2+ modulation system of myometrial contraction during gestation.In : Carfield RE and Tabb TN eds. Control of Uterine Contractility. Boca Raton, Ann Arbor, London, Tokyo ; CRC Press, 173-205 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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