1996 Fiscal Year Final Research Report Summary
Glycobiological Study of Malignant Tumors in Ophthalmology
| Project/Area Number |
07671928
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kagoshima University |
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Principal Investigator |
UEHARA Fumiyuki Faculty of Medicine Kagoshima University Associate Professor, 医学部, 助教授 (30168653)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGITA Toyoko University Hospital Kagoshima University Research Associate, 医学部・附属病院, 助手 (40271142)
SAMESHIMA Munefumi Faculty of Medicine Kagoshima University Research Associate, 医学部, 助手 (80041333)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Tumor / Glycobiology / Lectin / Sialyltransferase / Mucin / Ki 67 / Keratin |
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| Research Abstract |
The glycoconjugates in various malignant tumors in eyelids and orbita were analyzed by lectin histochemistry and in situ hybridization histochemistry using cRNA probes for sialiltransferases (ST). We considered that the cells, which expressed both cytoplasmic distribution of ST-mRNA and binding of lectins, specific for sialic acids, to the cell surfaces, were actively producing sialoglycans. We also considered that the cells, whose surfaces were stained with the lectins without cytoplasmic distribution of ST-mRNA,have completed the synthesis of sialoglycans. Based on these view points, this glycohistochemical technique is useful for dynamic analysis of the glycoconjugates in individual tumor cells. This technique revealed that O-linked sialoglycan, whose turnover-rate is slow, may be distributed over the cells of thickened spinocellular layr in the tumor of seborrheic keratosis and involved in its pathomechanism. We also analyzed the basal cell calcinoma in eyelids and the lacrimal pleomorphic adenoma using this method.
MUC 1, one of the O-linked glycoconjugates, was immunohistochemically detected in the tumor cells of all cases of squamous cell carcinoma and sebaceous carcinoma in eyelids whereas it was absent in those of all cases of basal cell carcinoma and benigh tumors in eyelids. These findings suggested that MUC 1 may be involved in expression of malignant features including metastasis. On the other hand, the Ki 67 antigen-expression rates of malignant eyelid tumors were higher than those of benigh tumor, suggesting that Ki 67 can be used to distinguish between malignant and benigh tumors. Immunohistochemical distribution of kearatin revealed that the basal cell carcinoma in eyelids is consisted of uniform tumor cells, all of which express the same keratin, while the squamous cell carcinoma and sebaceous carcinoma are composed of various differentiatiating tumor cells.
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