Analysis of clonality in odontogenic tumors by PCR assay of X chromosome gene

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07671969
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Osaka University
• Principal Investigator: FUKUDA Yasuo Osaka University Dental Hospital, Research-assistant, 歯学部・附属病院, 助手 (20238489)
• Co-Investigator(Kenkyū-buntansha): KISHINO Mitsunobu Osaka University Dental Hospital, Resident, 歯学部・附属病院, 医員 ; ISHIDA Takeshi Osaka University Dental Hospital, Associate-Professor, 歯学部・附属病院, 助教授 (50028768)
• Project Period (FY): 1995 – 1996
• Keywords: ameloblastoma / PCR / androgen receptor / clonality

Research Abstract

Ameloblastoma, the most popular odontogenic tumor, is classified as benign tumor. However, it shows infiltrative growth and destroy surrounding bony structure or soft tissues. Namely, "generally benign, but locally aggressive".
Recent advances in genomic technology enable the assesment of clonality in a lesion by PCR assay of X chromosome-linked gene. The aim of this study is to evaluate the clonality in ameloblastomas by PCR assay of human androgen receptor gene polymorphism.
From tissue-archive in our institute, 9 cases of ameloblastoma (all follicular type) arisen in female were selected as the specimens. Formalin-fixed, paraffin-embedded tissues were sliced and tumor area was isolated. The specimens were digested with proteinase-K and a part of them were digested with HpaII.The HpaII digested product and remaining undigested DNA were used as templates for PCR amplification for androgen receptor gene exonl including polymorphic trinucleotide repeat. The PCR product was loaded on polyacrylamide gel and phoresed. The gel was stained with silver staining.
Three of 9 cases did not have polymorhic allele. Remaining 6 cases showed two bands on the gel so that were evaluated as polyclonal.