1995 Fiscal Year Annual Research Report

口腔レンサ球菌のリポタイコ酸の免疫・生物学的活性

Research Project

Project/Area Number	07671977
Research Institution	Kagoshima University
Principal Investigator	高田春比古鹿児島大学, 歯学部, 教授 (30135743)
Co-Investigator(Kenkyū-buntansha)	川畑義裕鹿児島大学, 歯学部, 助手 (70274842) 新垣理恵子鹿児島大学, 歯学部, 助手 (40231120)
Keywords	口腔レンサ球菌 / Streptococcus sanguis / リポタイコ酸 / 線維芽細胞 / 肝細胞増殖因子 / インターロイキン-1 / 上皮細胞 / スキャッターファクター
Research Abstract	種々のグラム陽性菌の細胞表層に分布する両親媒性のリポタイコ酸(LTA)は,グラム陰性菌外膜を構成する内毒素性リポ多糖(LPS)に対応する構造物で,両者は共通の生物活性を示すとの説がある.そこで,我々は口腔レンサ球菌のLTA,特にデンタルプラークから最も高頻度に分離されるStreptococcus sanguis LTAに着目して,歯周組織のサイトカインネットワークに及ぼす作用を検討して,歯周病発症との係わりを探ろうとした. まず,Sigma社製の各種LTAを供試して,歯肉線維芽細胞培養に加えたところ,いずれのLTAも肝細胞増殖因子(HGF)/スキャッターファクター(SF)産生を増強した.特にS.sanguis LTAの活性は強かった.なお,対照とした各種LPSは全く活性を示さなかった.ちなみに,HGF/SFは上皮系細胞に多彩な作用を示すサイトカインとして注目を集めている.次に,歯肉上皮細胞にこれら標品を作用させて,HGF/SF誘導因子であるインターロイキン(IL)-1産生誘導作用を調べたところ,LPSは強い活性を示したが,LTAには活性は認められなかった.更に,リコンビナントヒト(rHu)IL-1αとS.sanguis LTAを併せて歯肉線維芽細胞に作用させると極めて強力にHGF/SFを誘導する事が明らかになった.この様な相乗作用は他のLTAでも認められた.なお,Sigma社製LTAは精製が不十分でLPS等の混入が認められた.従って,これまで報告のあるLTAによる各種サイトカイン誘導作用については再検討の必要があると考えられるが,本研究の成績は精製LTAでも確認されている.最後に,rHuHGFを歯肉上皮細胞に作用させると,期待通り,形態変化と増殖促進作用が認められた. 以上の成績は口腔に最も多数生息する口腔レンサ球菌のLTAが歯周組織を構成する細胞を直接・間接に活性化して組織破壊と治癒の両面で作用する可能性を示唆している.

Research Products
(15 results)

All Other

All Publications (15 results)

[Publications] H.Takada: "Molecular and structural requirements of a lipoteichoic acid from Enterococcus hirae ATCC 9790 for cytokine-inducing,antitumor,and antigenic activities." Infection and Immunity. 63. 57-65 (1995)
[Publications] K.Matsushita: "Immunopathological activities of extracellular products of Streptococcus mitis,particularly a superantigenic fraction." Infection and Immunity. 63. 785-793 (1995)
[Publications] Y.Aida: "Lipopolysaccharides from periodontal pathogens prime neutrophils for enhanced respiratory burst:differential effect of a synthetic lipid A precursor IV_A(LA-14-PP)." Journal of Periodontal Research. 30. 116-123 (1995)
[Publications] S.Nagao: "Modification of delayed-type hypersensitivity reactions by muramyldipeptide in guinea pigs." FEMS Immunology and Medical Microbiology. 11. 231-246 (1995)
[Publications] Y.Suda: "Cytokine-inducing glycolipids in the lipoteichoic acid fraction from Enterococcus hirae ATCC 9790." FEMS Immunology and Medical Microbiology. 12. 97-112 (1995)
[Publications] S.Nagao: "Endotoxin induces severe inflammatory reactions with necrosis at sites primed with delayed-type hypersensitivity reactions in guinea pigs." Microbiology and Immunology. 39. 1011-1014 (1995)
[Publications] Y.Aida: "An analogue of lipid A and LPS from Rhodobacter sphaeroides inhibits neutrophil responses to LPS by blocking receptor recognition of LPS and by depleting LPS-binding protein in plasma." Journal of Leukocyte Biology. 58. 675-682 (1995)
[Publications] S.Nagaoka: "Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prevotella intermedia lipopolysaccharide." Journal of Endodontics. 22. 9-12 (1996)
[Publications] H.Takada: "Structural characteristics of peptidoglycan fragments required to prime mice for induction of anaphylactoid reactions by lipopolysaccharides." Infection and Immunity. (in press). (1996)
[Publications] H.-O.Ito: "Lipopolysaccharides from Porphyromonas gingivalis,Prevotella intermedia,and Actinobacillus actinomycetemcomitans promote osteoclastic differentiation." Archives of Oral Biology. (in press). (1996)
[Publications] K.Matsushita: "Induction of lymphocytes cytotoxic to oral epithelial cells by Streptococcus mitis superantigen." Journal of Dental Research. (in press). (1996)
[Publications] A.Sugiyama: "Lipoteichoic acid and interleukin 1 stimulate synergistically production of hepatocyte growth factor/scatter factor in human gingival fibroblasts in culture." Infection and Immunity. (in press). (1996)
[Publications] D.E.S.Stwart-Tull(ed.): "The Theory and Practical Application of Adjuvants" John Wiley & Sons, 380 (1995)
[Publications] 中野昌康他(編): "エンドトキシン-新しい治療・診断・検査" 講談社, 327 (1995)
[Publications] J.Levin et al.(ed.): "Bacterial Endotoxins:Lipopolysaccharides from Genes to Therapy" Wiley-Liss,Inc., 608 (1995)

1995 Fiscal Year Annual Research Report

口腔レンサ球菌のリポタイコ酸の免疫・生物学的活性

Principal Investigator

高田 春比古 鹿児島大学, 歯学部, 教授 (30135743)

Research Products

[Publications] H.Takada: "Molecular and structural requirements of a lipoteichoic acid from Enterococcus hirae ATCC 9790 for cytokine-inducing,antitumor,and antigenic activities." Infection and Immunity. 63. 57-65 (1995)

[Publications] K.Matsushita: "Immunopathological activities of extracellular products of Streptococcus mitis,particularly a superantigenic fraction." Infection and Immunity. 63. 785-793 (1995)

[Publications] Y.Aida: "Lipopolysaccharides from periodontal pathogens prime neutrophils for enhanced respiratory burst:differential effect of a synthetic lipid A precursor IV_A(LA-14-PP)." Journal of Periodontal Research. 30. 116-123 (1995)

[Publications] S.Nagao: "Modification of delayed-type hypersensitivity reactions by muramyldipeptide in guinea pigs." FEMS Immunology and Medical Microbiology. 11. 231-246 (1995)

[Publications] Y.Suda: "Cytokine-inducing glycolipids in the lipoteichoic acid fraction from Enterococcus hirae ATCC 9790." FEMS Immunology and Medical Microbiology. 12. 97-112 (1995)

[Publications] S.Nagao: "Endotoxin induces severe inflammatory reactions with necrosis at sites primed with delayed-type hypersensitivity reactions in guinea pigs." Microbiology and Immunology. 39. 1011-1014 (1995)

[Publications] Y.Aida: "An analogue of lipid A and LPS from Rhodobacter sphaeroides inhibits neutrophil responses to LPS by blocking receptor recognition of LPS and by depleting LPS-binding protein in plasma." Journal of Leukocyte Biology. 58. 675-682 (1995)

[Publications] S.Nagaoka: "Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prevotella intermedia lipopolysaccharide." Journal of Endodontics. 22. 9-12 (1996)

[Publications] H.Takada: "Structural characteristics of peptidoglycan fragments required to prime mice for induction of anaphylactoid reactions by lipopolysaccharides." Infection and Immunity. (in press). (1996)

[Publications] H.-O.Ito: "Lipopolysaccharides from Porphyromonas gingivalis,Prevotella intermedia,and Actinobacillus actinomycetemcomitans promote osteoclastic differentiation." Archives of Oral Biology. (in press). (1996)

[Publications] K.Matsushita: "Induction of lymphocytes cytotoxic to oral epithelial cells by Streptococcus mitis superantigen." Journal of Dental Research. (in press). (1996)

[Publications] A.Sugiyama: "Lipoteichoic acid and interleukin 1 stimulate synergistically production of hepatocyte growth factor/scatter factor in human gingival fibroblasts in culture." Infection and Immunity. (in press). (1996)

[Publications] D.E.S.Stwart-Tull(ed.): "The Theory and Practical Application of Adjuvants" John Wiley & Sons, 380 (1995)

[Publications] 中野昌康他(編): "エンドトキシン-新しい治療・診断・検査" 講談社, 327 (1995)

[Publications] J.Levin et al.(ed.): "Bacterial Endotoxins:Lipopolysaccharides from Genes to Therapy" Wiley-Liss,Inc., 608 (1995)

高田春比古鹿児島大学, 歯学部, 教授 (30135743)