1996 Fiscal Year Final Research Report Summary
Dentinogenesis imperfecta in transgenic mice expressing a mutated collagen gene
| Project/Area Number |
07671985
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
SHIKATA Hideo MEIKAI UNIVERSITY,SCHOOL OF DENTISTRY,INSTRUCTOR, 歯学部, 助手 (00170853)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Transgenic mouse / Collagen gene / Dentinogenesis imperfecta |
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| Research Abstract |
Dentinogenesis imperfecta is a common inherited dental disease in which the defects of dentine result in tooth fragility. This dentine disorder is often observed in osteogenesis imperfecta with mutations in the type I procollagen genes, but so far, no experiment that the mutations responsible for osteogenesis imperfecta phenotype develope a dentinogenesis imperfecta has been established. We have investigated morphological changes of a dentinogenesis in transgenic mice expressing a partially deleted gene for human type I procollagen. The most striking feature was found in the teeth from 3-month old mice similar to dentinogenesis imperfecta with naked-eye and microscopic appearances as evidenced by decreased predentine and dentine collagen fibrils, severe reduction in the number and size of the dentinal tubules and in the calcospherites, pronounced incremental growth lines, and the disturbance in mineral distribution in either predentin or dentin. The most interesting changes are the reduced number of odontoblasts and the disturbed odontoblast differentiation. Comparisons of mice in age from 18-day embryo to 3-month old demonstrated that there was increasing evidence of a dentinogenesis imperfecta as the mice grew older. The data demonstrate compelling evidence that mutations in the type I procollagen gene cause a form of dentinogenesis imperfecta and indicate that this transgene causes an alternation in the differentiation of the odontoblasts leading to a decrease in the number of odontoblasts and alternation in dentine structure which is manifested as dentinogenesis imperfecta.
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