The Role of Integrin beta1 in morphogenesis and the cell differentiation the of the Tooth Germ

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07671994
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: IWASE Mineyo Kanagawa Dental College, Oral Histology, Assistant professor, 歯学部, 助手 (30155048)
• Project Period (FY): 1995 – 1996
• Keywords: focal adhesion kinase / integrin beta1 / morphogenesis / differentiation / rat tooth germ / ラット歯胚

Research Abstract

Integrin receptors for extracellular matrix molecules are thought to play important roles in morphogenesis since they mediate aspects of embryonic cell adhesion and migration. Using immunohistochemistry, the localization of integrin beta1 and focal adhesion-associated protein tyrosine kinase (FAK) were examined in murine tooth germs. The immunoreactivity of integrin beta1 was increased in the distal ends of inner dental epithelium with the accumulation of fibronectin. FAK was expressed at unique differentiation stages in both odontoblast and ameloblast lineages. During this stage, odontoblasts and ameloblasts changed in shape and predentin formation started.
Furthermore, integrin beta1 translation arrest was accomplished by antisense phosphorothioate oligodeoxynucleotide (ODN). All of the control explants cultured showed a normal structure in the formation of enamel and dentin, as well as tooth germ observed in vivo. In contrast, the antisense-treated explants did not show the formation of enamel and dentin, although they showed the normal crown pattern of teeth.
These results suggests that the signal transduction induced by integrin take part in the mineralization of the developing tooth.