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1996 Fiscal Year Final Research Report Summary

Purification of Osteoclast Differentiation Factor produced by Epiphyseal Cartilage

Research Project

Project/Area Number 07672014
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

HIRAKI Yuji  Osaka Univ.Fac.of Dentistry, Assoc.Prof., 歯学部, 助教授 (40144498)

Co-Investigator(Kenkyū-buntansha) INOUE Hiroyuki  Osaka Univ.Fac.of Dentistry Hospital, Assist.Prof., 歯学部・附属病院, 講師 (90167271)
Project Period (FY) 1995 – 1996
KeywordsChondrocyte / Growth Differentiation Factor / Cartilage Matrix / Chondromodulin-II / Osteoclast / Cell Differentiation
Research Abstract

Endochondral bone formation is initiated by the formation of cartilage tissue. Soon after the cartilaginous mold is formed, chondrocytes in the central part of the mold become hypertrophic and calcified. Concomitantly with neovascularization in the calcified cartilage zone, the bone-cell precursors are recruited to the ossification center where the differentiated osteoclasts gradually replace cartilage into bone and excavate the bone marrow cavity. Therefore, calcified cartilage must be the place where the differentiated osteoclasts appear for the fist time during embryonic development. Here we examined a possibility that calcified cartilage may play a functional role for formation of the differentiated osteoclasts. We isolated grwoth-plate chondrocytes from young rabbits, and cultured. The culture media were conditioned for two days each on Day 6 (at the confluent stage), Day 22 (at the maturing stage), Day 42 (at the hypertrophic stage), Day 48 (at the early calcified stage), and Day 57 (at the heavily calcified stage), respectively. Stimulatory activity of the medium on osteoclastic differentiation was assessed by pit formation assay (Bone Min.17 : 347-359,1992) and TRAP assay (Blood 74 : 1295-1302,1989). The results clearly indicated that calcified chondrocytes secreted a factor stimulatory for osteoclastic differentiation. Then we found the similar osteoclast differentiation factor in the guanidine extracts of fetal bovine epiphyseal cartilage of developing bone. The active principle was purified, and found identical with chondromodulin-II (ChM-II). ChM-II markedly stimulated osteoclastic differentiation at the dose rage of 3-10 ng/ml, but it did not required the presence of vitamin D_3 for action.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Inoue, H.: "Effect of Platelet-Derived Growth Factor on Growth and Phenotypic Expression of Rabbit Chondrocytes in Culture" Dentistry in Japan. 32. 41-45 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shukunami, C.: "Chondrogenic Differentiation of Clonal Mouse Embryonic Cell Line ATDC5 In VItro : Differentiation-dependent Gene expression of Parathyroid Hormone (PTH)/PTH-related Peptide Receptor" J. Cell Biol.133. 457-468 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akiyama, H.: "1α, 25-Dihydroxyvitamin D_3 Inhibits Cell Growth and Chondrogenesis of a Clonal Mouse EC Cell Line, ATDC5" J. Bone Min. Res.11. 22-28 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraki, Y.: "A Novel Growth-Promoting Factor Derived from Fetal Bovine Cartilage, Chondromodulin-II : Purification and Its Amino Acid Sequence" J. Biol. Chem.271. 22657-22662 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 開 祐司: "腫瘍学" 中外医学社, 270-282 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 開 祐司: "組織培養の技術 第3版" 朝倉書店, 167-170 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.INOUE: "Effect of Platelet-Derived Growth Factor on Growth and Phenotypic Expression of Rabbit Chondrocytes in Culture" Dentist.Japan. 32. 41-45 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.SHUKUNAMI: "Chondrogenic differentiation of clonal mouse embryonic cell line ATDC5 in vitro ; Differentiation-dependent gene expression of parathyroid hormone (PTH) /PTH-related" J.Cell Biol.133. 457-468 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.AKIYAMA: "1alpha, 25-Dihydroxyvitamin D_3 Inhibits Cell Growth and Chondrogenesis of a Clonal Mouse EC Cell Line, ATDC5" J.Bone Mine.Res. 11. 22-28 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.HIRAKI: "A Novel Growth-Promoting Factor Derived from Fetal Bovine Cartilage, Chondromodulin-II : Purification and Its Amino Acid Sequence" J.Biol.Chem.271. 22657-22662 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.HIRAKI: Oncology. Chugai Igakusha, 270-282 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.HIRAKI: Technique of Tissue Cluture 3rd Edition. Asakura Shoten, 160-170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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