Cytotoxicity testing of dental materials with saliva chemical factors in a culture medium

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07672142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Osaka Dental University
• Principal Investigator: IMAI Koichi Osaka Dental University, Department of Biomaterials, Lecturer, 歯学部, 講師 (90103100)
• Project Period (FY): 1995 – 1996
• Keywords: cytotoxicity / in vitro / artificial saliva / cell recovery / dental metal / monomer / tissue model / RC2

Research Abstract

To examine the cell sustainable composition of a culture medium containing saliva factors, two kinds of artificial saliva (Greenwood, Salivate) and human saliva were used to cultivate cells (HEp-2, L-929, RC2), followed by cell recovery in a fresh medium. By adding artificial saliva to a medium at 45%, approximately seventy percent of the cells were recovered. Cytotoxicities of eight metallic and three polymeric prosthetic materials were then examined by the cell recovery rate using two cell lines (HEp-2, Gin-1) and tissue model (MATREX) with addition of artificial saliva and human saliva at 45% to medium in vitro. Titanium, silver palladium alloy and heat-cured resin were not cytotoxic. A cobalt chromium alloy, and tin silver alloy showed only slight cytotoxicity. Silver indium alloy, nickel chromium alloy and nickel copper gallium alloy had moderate cytotoxic effects. Copper and two self-curing resins had marked effects. No difference between the two cell lines was found. The present findings suggest that saliva / tissue model couples for cytotoxicity testing of dental materials may be useful, despite the fact that the combination did not fully support cellular cultivation.