1996 Fiscal Year Final Research Report Summary
Effect of site selective cAMP & its metabolite on human adenocarucinoma cell line
| Project/Area Number |
07672177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | University of Tokushima |
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Principal Investigator |
YOSHIDA Hideo University of Tokushima, School of Dentistry, Assosiate Professor, 歯学部, 助教授 (30116131)
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| Project Period (FY) |
1995 – 1996
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| Keywords | 8-chloro-aenosine 3' : 5'-monophosphate (8-Cl-cAMP) / 8-chloro-adensine / human adenocarcinoma cell line / cAMP-dependent protein kinase (PKA) / cell proliferation |
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| Research Abstract |
We examined the effects of 8-chloro-adenosine 3' : 5'-monophosphate (8-Cl-cAMP), site-selective analogue of cAMP,and its metabolite 8-chloro-adenosine (8-Cl-ad) on the proliferation of human adenocarcinoma cell line HSG cells and its derivative cell line HSG-AZA1, HSG-AZA3, and human adenoid squamou carcinoma cell line TYS cells in culture. Cultivation of these cells in thepresence of 8-Cl-cAMP (0.5muM) or 8-Cl-ad (lmuM) resulted in the growth inhibition and reduction of colony formation rates on plastic surface or in soft agar to under 10%. Whereas the morphological alteration of treated cells were not shown. Then, it was confirmed that the HSG cells treated with 8-Cl-cAMP (0.5muM) or 8-Cl-ad (1muM) were not induced apoptosis, because DNA pattern was similar to untreated cells in agar gel electrophoresis. In addition, the cAMP-dependent protein kinase A,isolated from treated HSG cells, were decreased.
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Research Products
(8 results)
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[Publications] Sato M,Kawamata H,Harada K,Nakashiro K,Ikeda Y,Gohda H,Yoshida H,et al: "Induction of cyclin-dependent kinase inhibitor, p21^<KAF:> by treatment with 3,4-dihydro-6-[4-(3,4)-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinon) in a human salivary cancer cell line with mutant p53 gene." Cancer Lett.(in press). (1997)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sato M,Kawamata H,Harada K,Nakashiro K,IKeda Y,Gohda H,Yoshida H,Nishida T,Ono K,Kinoshita M and Adachi M: "Induction of cyclin-dependent kinase inhibitor, p21^<WAP1>, by treatment with 3,4-dihydro-6-[4-(3,4)-dimethoxybenzoyl)-1-piperazinyl]-2 (1H)-quinolinone (vesnarinon) in a human salivary cancer cell line with mutant p53 gene" Cancer Lett. (in press).
Description
「研究成果報告書概要(欧文)」より
