1996 Fiscal Year Final Research Report Summary
Pharmacogenetics of drug metabolizing enzymes
| Project/Area Number |
07672337
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YOKOI Tsuyoshi Hokkaido Univ., Fac.of Pharm.Res.Associate Prof., 薬学部, 助教授 (70135226)
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| Co-Investigator(Kenkyū-buntansha) |
KAMATAKI Tetsuya Hokkaido Univ., Fac.of Pharm.Res.Prof., 薬学部, 教授 (00009177)
NAKAYAMA Katsuo Hokkaido Univ., Fac.of Pharm.Res.Instructor, 薬学部, 助手 (20261323)
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| Project Period (FY) |
1995 – 1996
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| Keywords | genotyping / SM-12502 / coumarin / poor metabolizer / polymorphism / CYP2A6 / population study |
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| Research Abstract |
Genetic polymorphism of CYP2A6 was investigates. (+) -cis-3,5-dimethyl-2- (3-pyridyl) thiazolidin-4-one hydrochloride (SM-12502) was found to be a specific substrate of CYP2A6. Genomic DNA form extensive metabolizers and poor metabolizers (PM) of SM-12502 were examined by Southern blot analysis with CYP2A6cDNA probe. A new Sac I-RELP which distinguish PM was investigated. Finally, we concluded that the PM of SM-12502 was responsible for CYP2A6 whole gene deletion. A new genotyping method for CYP2A6 whole gene deletion with PCR was developed.
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[Publications] Ken-ichi Nunoya, Tsuyoshi Yokoi, Kanzo Kimura, Takao Kodama, Masahito Funayama, Kazuaki Inoue, Kazuo Nagashima, Yoshihiko Funae, Noriaki Shimada, Carol Green and Tetsuya Kamataki: "(+) -cis-3,5-dimethyl-2- (3-pyridyl) thiazolin-4-one Hydrochloride (SM-12502) as a novel substrate for cytochrome P450 2A6 (CYP2A6) in human liver microsomes" J.Pharmacol.Exptl.Therap.277. 768-774 (1996)
Description
「研究成果報告書概要(欧文)」より
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