1996 Fiscal Year Final Research Report Summary
Genes those regulate internal osmolality
| Project/Area Number |
07672344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Chiba University |
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Principal Investigator |
NAKAMURA Tatsunosuke Chiba University・Faculty of Pharmaceutical Science・Research Associate, 薬学部, 助手 (20114308)
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| Project Period (FY) |
1995 – 1996
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| Keywords | trkA / mscL / nhaB / osmolality / gene |
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| Research Abstract |
(1) trkAv and neighboring genes.
We clone K^+ uptake genes, trkAv and trkHv, from marine bacterium, Vibrio alginolyticus, those functionally complement K^+ uptake deficient E.coli strains. K^+ uptake activities of those strains with trkAv and/or trkHv genes are determined. Expression of TrkAv and TrkHv were examined also.
(2) mscL gene.
We examined the activity of MscL in Vibrio alginolyticus.
(3) nhaBv gene.
We cloned nhaBv, the second Na^+/H^+ antiporter, gene from V.alginolyticus. Topological experiments were done by phoA gene fusion method. It was appeared that NhaBv has odd number transmembrane segments.
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