1996 Fiscal Year Final Research Report Summary
A study on function mechanism of a novel endogenous
| Project/Area Number |
07672481
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE |
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Principal Investigator |
HAZATO Tadahiko THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SIENCE,CANCER THERAPEUTICS,SCIENTIST, 化学療法研究部門, 研究員 (60109949)
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| Co-Investigator(Kenkyū-buntansha) |
KUGIMIYA Toyoki JUNTENDO UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部・麻酔科, 教授 (90010537)
YAMAMOTO Yukio THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SIENCE,CANCER THERAPEUTICS,SCIENTIST, 化学療法研究部門, 研究員 (80124501)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Enkephalin / Spinorphin / Aminopeptidase N / Leuhistin / Anti-inflammatory |
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| Research Abstract |
Spinorphin is a novel endogenous pain-regulated substance whic modulated an enkephalin-degrading activity. In this study, we found that spinorphin causes a significant inhibition of inflammatory responses by human PMNs stimulated with FMLP and may be a new endogenous anti-inflammatory peptide which is mediated in part through the suppression in [Ca^<2+>] i changes. Next we examined the effects of spinorphin, using an in vivo system involving mouse air-pouch as a model of acute inflammatory response. In this model carrageenan and PMNs accumulation at inflammatory sites was an indicator of an acute inflammatory response. Intravenous administration of spinorphin within air-pouch site elicited by carrageenan, the inhibition being 64%, compared to the saline-treated group. Combination of leuhistin, an inhibitor of aminopeptidase N,significantly suppressed PNMs accumulation induced carrageenan. In the future, spinorphin will be an important tool for use in clarifying of neuropeptide-induced biological phenomena such as enkephalin mediated pain and inflammation.
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