1996 Fiscal Year Final Research Report Summary
Effects of Antigenic Composition of Calibrator and Serum on Lipoprotein (a) Measurement
| Project/Area Number |
07672497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Jichi Medical School |
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Principal Investigator |
SAKURABAYASHI Ikunosuke Jichi Medical School Chairman, Professor, 医学部, 教授 (80049056)
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| Co-Investigator(Kenkyū-buntansha) |
KUBO Nobuhiko Jichi Medical School assistant, 医学部, 助手 (40214994)
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| Project Period (FY) |
1995 – 1996
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| Keywords | lipoprotein (a) / apo lipoprotein (a) / immunoassay / monoclonal antibody / standardization |
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| Research Abstract |
Two direct binding ELISA using anti-Lp (a) /apo (a) antibody was developed to investigate the effects of calibration on Lp (a) value. Paired Lp (a) value of sera from 59 individuals was obtained by the identical ELISA using two calibration curves by each commercial calibrator. The difference between paired Lp (a) value was produced by the reactivity [against the anti-Lp (a) /apo (a) antibody] difference among constructs of the two calibrators. Not only the reactivity against anti-Lp (a) /apo (a) antibody, but also the amount of partial Lp (a) antigen were different among constructs and between the two calibrators suspected that the disagreements between the paired Lp (a) value by the two calibrators could be delived from different transferred Lp (a) value from standards to each constructs of calibrator. We also found the partial Lp (a) antigen was heterogeneous among individuals that may affects on Lp (a) value among different Lp (a) assays. Even it is not complete but it was suspected that not only unifying but also opening the character of standard material and acheive the agreement over the method for transferring Lp (a) value from standard to the calibrator of each assay, are neccessary to reduce the difference in Lp (a) values by different assay methods.
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