• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Purification of protease induced by ultraviolet ray in human cells.

Research Project

Project/Area Number 07680571
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionChiba University

Principal Investigator

SUZUKI Nobuo  Chiba university, Medicine, Professor, 医学部, 教授 (90111426)

Project Period (FY) 1995 – 1996
KeywordsProtease / Ultraviolet ray / Human cell
Research Abstract

Search for protease which supervises ultraviolet ray (UV)-response mechanisms in human cells was made. First, partial purification of protease itself was done by establishment of affinity chromatography. Second, several genes, which may be related with protease and/or its metabolism, were detected by differential display methods in cell lines with and without protease inducibility after UV.Thus, we could find out the methods for obtaining protease or its-related genes, although it is required for us to make more efforts.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Suzuki,N.et al: "Suppression of saccharin-induced mutagenicity by interferon-α in human RSa cells" Cancer Res.55. 4253-4256 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.et al: "Dipyridamole combined with tumor necrosis factor-α enhances inhibition of proliferation in human tumor cell lines" Jpn.J.Cancer Res.86. 761-769 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.et al: "Piperonyl butaxide mutagenicity in human RSa cells" Mutation Res.344. 27-30 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugita,K.et al: "Reduction of unscheduled DNA synthesis and plasminogen activator activity in Hutchinson-Gilford fibroblasts during passagina in ritro" Mulation Res.316. 133-138 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishizuka,T.et al: "Promoter region of the mouse cyclin-dependent kinase 5-encoding gene" Gene. 166. 267-271 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inaba,N.et al: "Cytokeratin fragment 21-1 in gynecologic malignancy : Comparison with cancer antigen 125 and squamous cell carcinoma-related antigen" Tumor Biol.16. 345-352 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.et al: "Suppression of UV-and interferon α-refractoriness by antipain in human IF^r cells established from RSa cells sensitive to both stimuli" J.Cell Physiol.167. 47-51 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishizuka,T.et al: "Cloning and sequencing of human complementary DNA for the phosphoriboryl pyrophosphate synthetase-associated protein 39" Biochim.Biophys.Acta. 1306. 27-30 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.et al: "Hypomutabll potential accompanying interferon-α-resistance in a human cell line,IF^r,derived from interferon-α-sensitive RSa cells" J.Interferon Cytokine Res.16. 733-738 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.et al: "Correlated susceptibility between interferon-β and VV in human cell lines,F-IF^r and RSa" Int,J.Oncol.9. 297-303 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.et al: "K-ras codon 12 mutation induction by VV but not by 4NQD,analyzed by PCR following differential dot-blot hybridization,in hypermutable human RSa cells" Environ.Mutagen.Res.18. 29-34 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugita,K.et al: "Involvement of antipain-sensitie protease activity in the interferon-β-induced VV-refractoriness of Cockayne syndrome fibroblasts" Mutation Res.357. 177-181 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishizuka, T., Ahmad, I., Kita, K., Sonoda, T., Ishijima, S., Sawa, K., Suzuki, N.and Tatibana, M.: "The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25." Genomics. 33. 332-334 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N.: "Suppression of UV- and interferon-alpha-refractoriness by antipain in human IFr cells established from RSa cells sensitive to both stimuli." J.Cell.Physiol.167. 47-51 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishizuka, T,Kita, K., Sonoda, T., Ishijima, S., Sawa, K., Suzuki, N.and Tatibana, M.: "Cloning and sequencing of human complementary DNA for the phosphoribosylpyrophosphate synthetase-associated protein 39." Biochim.Biophys.Acta.1306. 27-30 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishizuka, T., Kita, K., Sawa, K., Sonoda, T., Suzuki, N.and Tatibana, M.: "Promotor region of the rat phosphoribosylpyrophosphate synthetase-associated protein 39." Biochim.Biophys.Acta.1306. 34-37 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N., Suzuki, H., Ishizuka, T., Yamamori, H.and Takakubo, Y.: "Hypomutable potential accompanying interferon-alpha-resistance in a human cell line, IF^r, derived from interferon-alpha-sensitive and hypermutable RSa cells." J.Interferon Cytokine Res.16. 733-738 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N., Iida, S., Yamamori, H., Ishizuka, T.and Takakubo, Y.: "Correlated susceptibility between interferon-beta and UV in human cell lines, F-IF^r and RSa : implication of unifying antipain-sensitive mechanisms for both stimuli." Int.J.Oncol.9. 297-303 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N., Suzuki, H., Ishizuka, T.and Yamamori, H.: "K-ras codon 12 mutation inductin by UV but not by 4NQO,analyzed by PCR following differential dot-blot hybridization, in hypermutable human RSa cells." Environ.Mutagen Res.18. 29-34 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita, K., Suzuki, N.and Niimi, H.: "Involvement of antipain-sensitive protease activity in the inerferon-beta-induced UV-refractoriness of Cockayne syndrome fibroblasts." Mutat.Res.357. 177-181 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi