1996 Fiscal Year Final Research Report Summary
Design, structures and functions of stabilized artificial ion channels
| Project/Area Number |
07680629
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IIDA Akira Kyoto Univ., Fac.of Pharm.Sci., Associate Professor, 薬学部, 助教授 (40202816)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAOKA Yasuo Kyoto Univ., Fac.of Pharm.Sci., Instructor, 薬学部, 助手 (90243039)
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| Project Period (FY) |
1995 – 1996
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| Keywords | artificial ion channel / peptide bunndle / Trichoderma polysporum / trichosporin / peptaibol / voltage-dependent channel / imperfect fungus / barrel stave |
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| Research Abstract |
20-residue peptaibols, trichosporins-B produced by the fungus Trichoderma polysporum, forms voltage-dependent ion channels in planar lipid bilayr membranes. To clarify primary factors for channel stabilization and to develop new functional materials such as artificial ion channels, trichosporin derivatives including a template-assembled dimer and tetramer and two disulfide-linked dimers were synthesized and their channel-forming properties were investigated.
The results obtained in this study were summarized as followes ;
1.The chennels induced by truncated and elongated derivertives were unstable, suggesting that peptide length fitting the membrane thickness is quite important.
2.The Pro-kinked structure is not essential for voltage-dependent channel formation and very important for forming stable channels with large pore size.
3.Peptide lipophilicity promotes peptide aggregation and this is not in accordance with channel stability.
4.Stability of channels is influenced by the states of supermolecules consisting of pepides, lipids and water.
5.All chemically assembled peptides (helix bundle) form very long-lasting channels, supporting that peptaibol channels can be explained through the barrel stave model.
6.Success in formation of the stable channels may lead to developing novel functional materials.
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