1997 Fiscal Year Final Research Report Summary
Formation of hyaluronic-acid-deficient extracellular matrix induced by an inhibitor for hyaluronic acid synthesis
| Project/Area Number |
07680668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
NAKAMURA Toshiya School of Allied Medical Sciences, Associate Professor, 医学部, 助教授 (00155847)
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| Co-Investigator(Kenkyū-buntansha) |
MUNAKATA Hidekazu School of Medicine, Research Associate, 医学部, 助手 (80271807)
TAKAGAKI Keiichi School of Medicine, Associate Professor, 医学部, 助教授 (70163160)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Hyaluronic acid / Inhibitor / Extracellular matrix / Human skin fibroblasts / 4-Methylumbelliferone / Decorin |
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| Research Abstract |
Cultivation of human skin fibroblasts in the presence of 0.5 mM 4-methylumbelliferone repressed hyaluronan production of the cell and reduced its chain length, resulting establishment of hyaluronan-deficient extracellular matrix. Cell-free experiments suggested that 4-methylumbelliferone may repress the expression of hyaluronan synthase on the cell surface. Electron-microscopic analysis of the hyaluronan-deficient extracellular matrix showed decrease of extracellular matrix component morphologically. Analysis of hyaluronan-binding proteins in medium showed the decrease of the amount of hyaluronan binding proteins, especially 83kDa protein, in the medium of the cells cultured in the presence of 4-methylumballiferone. Synthesis of proteoglycans of the cells were not affected by 4-methylumbelliferone signifcantly except for that of decorin. The synthesis of decorin was stimulated by 4-methylumbelliferone and, moreover, the number of glycosaminoglycan chains of decorin was increased from 1 to 2 by 4-methylumbelliferone.
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