1996 Fiscal Year Final Research Report Summary
Isolation, characterization of represser protein in expression of human DLST gene.
| Project/Area Number |
07680694
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Fitness & Sports in Kanoya |
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Principal Investigator |
MATUDA Sadayuki Faculty of Physiological Exercise National Institute of Fitness & Sports in Kanoya Professor, 体育学部, 教授 (40041371)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Kyoko Department of Biochemistry, Kagoshima Women's Junior College, Professor, 教授 (70094159)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Dihydrolipoamide succinyltransferase / alpha-ketoglutarate dehydrogenase complex / regulation of gene expression / analysis of function of promoter region |
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| Research Abstract |
In this study, we analyzed the function in expression of human dihydro lipoamide succinyltransferase gene. The enzyme is a core-component of 2-oxoglutarate dehydrogenase complex and plays a role in energy production in mitrochondria. We have isolated the genomic DNA for the DLST gene and determined the entire nucleotide sequence. Sequence analysis of the promoter-regulatory region of the DLST gene showed the presence of a CAAT-box-like sequence but the presence of a TATA-box-like sequence was not evidenced. Also located in this region were sequences resembling glucocorticoid-responsive and cAMP-responsive elements, and an Spl binding site. Thus, the DLST gene is a typical housekeeping gene. Gene group of this type, possessing multiple GC boxes but no TATA box, is activated by transcription factor Spl or Spl-related proteins. In this study, however, we found that the DLST gene is not activated by Spl or Spl-related proteins. A region-35 to-12 upstream from the transcription site is required for the transcription of the DLST gene. Furthermore, we found that the a region +796 to +964 in the second intron strongly supresses the expression of the DLST gene. The DLST gene may be regulated by the combination of the transcrption-activation region-35 to-12 and of the transcription-supression region +796 to +964.
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