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1996 Fiscal Year Final Research Report Summary

FUNCTION AND EXPRESSION-REGULATION OF DROSOPHILA DNA REPLICATION ENZY ME GENE

Research Project

Project/Area Number 07680704
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionAICHI CANCER CENTER RESEARCH

Principal Investigator

YAMAGUCHI Masamitsu  AICHI CANCER CENTER RESEARCH INSTITUTE,LABORATORY OF CELL BIOLOGY,SECTION HEAD, 研究所・生物学部, 室長 (00182460)

Project Period (FY) 1995 – 1996
KeywordsPCNA / Drosophia / DNA replication enzyme gene / transcriptional regulatory element / transcription factor / transgenic animal
Research Abstract

1. Analyzes of transcriptional regulatory elements of Drosophila PCNA gene using transgenic files : We have established more than 150 independent lines of transgenic files carrying PCNA promoter-lacZ fusion genes having various mutations in the PCNA promoter. Examination of lacZ expression from these transgenic lines during development allowed us to identify three transcriptional regulatory elements (URE : upstrem regulatory elemet, DRE : DNA replication-related element and E2F-recongnition sites). The results indicate that E2F-recognition sites are eddectial for the PCNA gene expression throughout development. DRE is essential in embryos and larvae. URE is essential in various larval tissues.
2. Analyzes of functions of regulatory factors for DNA replication enzyme genes using Gal4-targetted expression system : Overexpression of DREF (DRE-binding factor) in Drosophila eye imaginal discs results in abnormal morphogenesis of complex eyes in adolt fly. This is likely caused by ectopic induction of cell proliferation in eye imaginal discs, although detailed machanisms are now under investigation. Overexpression of dominant-negtive form of DRFE in salivary glands inhibited the polytenization of chromosomes. The results indicate that DREF plays an important role in regulation of endoreplization cycles.
3. Identification of DREF homologue in species other than Drosophila : Immunoscreeming of Bombyx mori. cDNA library using anti-Drosophila DREF antibody allowed us to pick up several candidate clones for silk worm homologue of DEEF.We are now analyzing nucleotide sequences of these candiate clones. Comparison of structures of DREF between these two species would hopefuly prvide us a clue to identify mammalian homologue of DREF.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Yamaguchi,M.et al.: "Role of homeodomain protein-binding region in the expression of..." Gene Expression. 4. 183-193 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,M.et al.: "A nucleotide soquence essential for the function of DRE..." J.Biol.Chem.270. 15808-15814 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,M.et al.: "Essential role of E2F recognition sites in regulation of..." J.Biol.Chem.270. 25159-25165 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,M.et al.: "Roles of multiple pronoter elements of the proliferating..." Genes to Cells. 1. 47-58 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,M.et al.: "Expression patterns of DNA replication enzymes and..." Biol.Cell. 85. 147-155 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirose,F.et al.: "Isolation and characterization of cDNA for DREF..." J.Biol.Chem.271. 3930-3937 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口政光: "DNA複製・修復と発癌" 羊土社, 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi, M., Nishida, Y.and Matsukage, A.: "Role of homeodomain protein-binding region in the expression of Drosophola proliferating cell nuclear antigen gene : Analysis with transgenic flies" Gene Expression. 4. 183-193 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, M., Hayashi, Y., Nishimoto, Y., Hirose, F.and Matsukage, A.: "A nucleotide sequence essential for the function of DRE,a common promoter element for Drosophila DNA replication-related genes" J.Biol. Chem.270. 15808-15814 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, M., Hayashi, Y.and Matsukage, A.: "Essential role of E2F recognition sites in regulation of the proliferating cell nuclear antigen gene promoter during Drosophila development" J.Biol. Chem.270. 25159-25165 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsukage, A., Hirose, F., Hayashi, Y., Hamada, K.and Yamaguchi, M.: "The DRE sequence TATCGATA,a putative promoter-activating element for Drosophila melanogaster cell proliferation-related genes" Gene. 166. 233-236 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, M., Hirose, F.and Matsukage, A.: "Roles of multiple promoter elements of the proliferating cell nuclear antigen gene diring Drosophila development" Genes to Cells. 1. 47-58 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, M., Hirose, F., Nishimoto, Y., Naruge, T., Ikeda, M., Hachiya, T., Tamai, K., Kuroda, K.and Matsukage, A.: "Expression patterns of DNA replication enzymes and the regulatory factor DREF during Drosophila development analyzed with specific antibodies" Biol. Cell. 85. 147-155 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose, F., Yamaguchi, M., Kuroda, K., Omori, A., Hashiya, T., Ikeda, M., Nishimoto, Y., and Matsukage, A.: "Isolation and characterization of cDNA for DREF,a promoter-activating factor for Drosophila DNA replication-related genes" J.Biol. Chem.271. 3930-3937 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, Y., Yamagushi, M., Hirose, F., Cotterill, S., Kobayashi, J., Miyajima, S.and Matsukage, A.: "DNA replication-related elements cooperate to enhance promoter activity of the Drosophila DNA polymerase alpha 73-kDa subunit gene" J.Biol. Chem.271. 14541-14547 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryu, J.-R., Choi, T.-Y., Kwon, E.-J.., Lee, W.-H., Nishida, Y., Hayashi, Y., Matsukage, A., Yamaguchi, M.and Yoo, M.-A.: "Transcriptional regulation of teh Drosophila raf protooncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system" Nucleic Acids Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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