1996 Fiscal Year Final Research Report Summary

Mating Behavior of Zebra Fish and Related Neural Network

Research Project

Project/Area Number	07680726
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Biophysics
Research Institution	OKAYAMA University
Principal Investigator	NAKAYASU Hiyoshi Okayama University, Dept.of Biology, Associate Professor, 理学部, 助教授 (60135465)
Project Period (FY)	1995 – 1996
Keywords	Zebra-fish / Brain / Neural Network / Mating Behavior / Monoclonal Antibody / Immuno staining
Research Abstract	A monoclonal antibody was prepared using adult zebrafish brain synaptic membrane proteins as antigen. The monoclonal antibody recognized only one specific protein (molecular weight was about 100kDa) on western blot analyzes using zebrafish brain synaptic membrane proteins. However, this monoclonal antibody did not recognized any other proteins from non neuronal tissues. The antibody stained bundle of neural axons in zebrafish brain, such as optic, octaval and vagal nerves. The antibody also stained oligodendrocytes in optic nerve, and myelin layr of Mauthner axon which exists as extremely big and very visible axon in zebrafish. Therefore, these results, taken together, indicated that this monoclonal antibody specifically recognized oligodendrocytes in zebrafish central neural system.