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1996 Fiscal Year Final Research Report Summary

Mating Behavior of Zebra Fish and Related Neural Network

Research Project

Project/Area Number 07680726
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOKAYAMA University

Principal Investigator

NAKAYASU Hiyoshi  Okayama University, Dept.of Biology, Associate Professor, 理学部, 助教授 (60135465)

Project Period (FY) 1995 – 1996
KeywordsZebra-fish / Brain / Neural Network / Mating Behavior / Monoclonal Antibody / Immuno staining
Research Abstract

A monoclonal antibody was prepared using adult zebrafish brain synaptic membrane proteins as antigen. The monoclonal antibody recognized only one specific protein (molecular weight was about 100kDa) on western blot analyzes using zebrafish brain synaptic membrane proteins. However, this monoclonal antibody did not recognized any other proteins from non neuronal tissues. The antibody stained bundle of neural axons in zebrafish brain, such as optic, octaval and vagal nerves. The antibody also stained oligodendrocytes in optic nerve, and myelin layr of Mauthner axon which exists as extremely big and very visible axon in zebrafish. Therefore, these results, taken together, indicated that this monoclonal antibody specifically recognized oligodendrocytes in zebrafish central neural system.

  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Nakayasu,H.: "Cerebral GABA_A and GABA_B veceptors : Stracture and function" Annals New York Acad.Sci.757. 516-527 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayasu,H.: "Crystal Violet as an indicator dye for non equili briam pH gradiene electro phoesis (NEPHQE)" Anal.Biochem.229. 259-262 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中安博司: "生物薬科学実験講座第12巻" 広川書店(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayasu, H.: "Cerebral GABA_A and GABA_B receptors : Structrure and function." Anals New York Acad.Sci.757. 516-527 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayasu, H.: "Crystal Violet as an indicator dye for nonequilibrium pH gradient electrophoresis (NEpHGE)." Anal.Biochem.229. 259-262 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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