1996 Fiscal Year Final Research Report Summary

Identification of transducer molecules for BMP signaling

Research Project

Project/Area Number	07680733
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Molecular biology
Research Institution	HOKKAIDO UNIVERSITY
Principal Investigator	SHIBUYA Hiroshi Hokkaido Univ., Fac.of Pharm.Sci.Associate Prof., 薬学部, 助教授 (30261324)
Project Period (FY)	1995 – 1996
Keywords	BMP receptor / Signal transducers / Two-hydrod system / BRAM2 / Target gene / Differential dispaly / Dlx-5
Research Abstract	1. Identification of signal transducers binding to the cytoplasmic region of BMP type I receptor (BMPR-I). To clarify BMP receptor mediated signaling, the cytoplasmic interactors cDNAs of BMPR-I,which includes serine-threonine kinase, were isolated from human placenta cDNA library with the ose of a yeast two-hydrid system. The isolated 4 clones were termed to BRAM1-4 (BMP receptor associated molecule 1-4). We confirmed that BRAM2 interacted to catalytic domain of BMPR-I in yeast and mammalian cells. Moreover, BRAM2 also interacted to TGF-beta type I receptor. 2. Identification of BMP-inducing molecules cDNA.To identify target genes for BMP signaling, we performed Differential display method using the mRNA prepared from MC3T3-E1 cells with or without BMP-stimulation, and Dlx-5 gene, which contains a homeotic box, were isolated. We confirmed that Dlx-5 gene was expressed by BMP-stimulation. The stable cell line which overexpressed Dlx-5, seems to lead the bone differentiation.