1996 Fiscal Year Final Research Report Summary
STRUCTURAL AND FUNCTIONAL ANALYSIS OF A NEW CANDIDATE TUMOR-SUPPRESSOR GENE,DAN
| Project/Area Number |
07680754
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | CHIBA CANCER CENTER RESEARCH INSTITUTE |
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Principal Investigator |
OZAKI Toshinori CHIBA CANCER CENTER RESEARCHINSTITUTE, 研究局・生化学研究部, 研究員 (40260252)
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| Project Period (FY) |
1995 – 1996
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| Keywords | DAN / DA41 / Two-hybrid |
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| Research Abstract |
(1) Characterization of the human DAN gene
The human genomic DNA,which contains the entire region of DAN gene, has been cloned from the standard phage- and the P1-based genomic libraries and its nucleotide sequence was determined. The human DAN gene is composed of four exons and there exist the possible CA-repeats within the first intron. Importantly, allelic loss of DAN gene locs was observed in three neuroblastoma tissues with N-myc gene amplification. At present, the molecular basis of the structural abnormalities of DAN gene in some of the neuroblastomas is not known.
(2) ldentification of a new cellular protein which can associate with DAN
By using a yeast two-hybrid screening, a new cellular protein (DA41) which can interact with DAN was identified. The DA41 cDNA encodes a new protein compised of 582 amino acids. The expression of DA41 gene was regulated in a cell cycle-dependent manner, raising a possibility that DA41 might possess a cell cycle-reglatory role. When DA41 gene was overexpressed in ras-transformed 3Y1 cells, phenotypic reversion was observed.
Interestingly, the amount of WAF1 was increased and the activity of CDK2 was significantly reduced in these DA41-overexpressing cells. These results strongly suggest that the DAN-DA41 complex could contain a significant role in a cell cycle regulation.
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[Publications] Ozaki, T., Hishiki, T., Toyama, Y., Yasa, S., Nakagawara, A.and Sakiyama, S.: "ldentification of a new cellular protein which can interact with DAN." DNA and Cell Biol.(in press). (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ozaki, T.Enomoto, H., Nakamura, Y., Kondo, K., Seki, N., Ohira, M., Nomura, N., Ohki, M., Nakagawara, A.and Sakiyama, S.: "The genomic analysis of human DNA gene." DNA and Cell Biol. (in press). (1997)
Description
「研究成果報告書概要(欧文)」より
