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1996 Fiscal Year Final Research Report Summary

Analysis of cell cycle controlled by Rb tumor suppresser gene

Research Project

Project/Area Number 07680756
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionTohoku University

Principal Investigator

IKAWA Shuntar  Institute of Development, Aging and Cancer, Tohoku University Assistant professor, 加齢医学研究所, 助手 (50241576)

Project Period (FY) 1995 – 1996
Keywordstumor suppresser gene / RB gene / differential display / inducible expression / cell cycle
Research Abstract

Exogenous expression of Rb tumor suppresser gene in cells was formerly difficult due to its intrinsic nature of inhibiting cell growth. However, the use of inducible expression system led to isolation of derivatives of NIH3T3 cell line, which will undergo complete growth arrest upon induction of RB.Incidentally, all of the information necessary for an organism is preserved as a genetic material, DNA.Therefore, realization of the information is fundamental processes of life, especially transcription step should deserve special attention. Differential display method (DD) is the most efficient and consistent method currently available for displaying transcripts present in the cells. The RB gene is speculated to exert its function by interacting with various transcriptional machinery, thus outcome of its action must be reflected in alteration of transcription. I have further modified original DD more consistent and efficient resulting in improved DD.By applying the method to RB cells, a number of genes emerged that manifested itself to be enhanced or reduced expression following RB induction. We are currently in the process of analyzing these genes. Parenthetically, we are also looking into genes involved in serum starvation, serum stimulation, contact inhibition, senescence, differentiation, and apoptosis by the same method. An extensive survey of these genes will enable us to classify genes involved in general growth arrest processes or more specialized growth arrest processes leading to discovery of more definitive and unambiguous definition of function of these genes involved in growth arrest.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kikuchi,H.,: "Different inducibility of cytochrome P-450LA1 mRNA of human and mouse by omeprazole in culture cells." Arch.Biochem.Biophys.316. 649-652 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikawa,S.,: "Assessment of Cancer Susceptibility in Humans by Use of Genetic Polymorphisms in Carcinogen Metabolism." Pharmacogenetics. 5. 154-160 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hossain,A.,: "Identification of cellular proteins that can interact specifically with the bHLH domain of the Ah receptor." Biochem.Biophys.Res.Com.215. 405-411 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hossain,A.: "Identification of a 120 kDa protein associated with aromatic hydrocarbon receptor nuclear translocator." Biochem.Biophys.Res.Com.212. 144-150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ema,M.: "Two new members of the murine Sim gene family are transcriptional repressors and show different expression patterns during mouse embryogenesis." Mol.Cell.Biol.16. 5867-5875 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikuchi,H.: "The different inducibility of CYP1A1-mRNA between the human and the mouse cells by benzimidazole compounds." Arch.Biochem.Biophys.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井川俊太郎: "Medical lmmunology特集/Cell-cycle,28 Rb1と細胞周期" 国際医書出版, 336 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井川俊太郎: "組織培養 特集:癌関連遺伝子とその関連領域の研究の最近の進歩 Rb遺伝子:その後の展開" ニューサイエンス社(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikawa, S., Uematsu, F., Watanabe, K., Kimpara, T., Osada, M., Hossain, A., Sagami, I., Kikuchi, H., and Watanabe, M: "Assessment of tumor susceptibility of the humans by use of genetic polymorphisms in carcinogen metabolisms." Pharmacogenetics. 5. S154-S160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikuchi, H., Hossain, A., Sagami, I., Ikawa, S., and Watanabe, M.: "Different inducibility of cytochrome P450IA1 mRNA of human and mouse by omeprazole in culture cells." Arch.Biochem. Biophys.316(1). 649-652 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hossain, A., Kikuchi, H., Ikawa, S., Sagami, I., and Watanabe, M.: "Identification of a 120 KDa protein associated with aromatic hydrocarbon receptor nuclear translocator" Biochem. Biophys. Res.Commun.212(1). 144-150 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hossain, A., Kikuchi, H., Ikawa, S., Sagami, I., and Watanabe, M.: "Identification of cellular protein that can interact specifically with the basic helix-loop-helix domain of the aromatic hydrocarbon receptor." Biochem. Biophys. Res.Commun. 215(1). 405-411 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ema, M., Morita, M., Ikawa, S., Tanaka, M., Matsuda, Y., Gotoh.O., Saijyo, Y., Fujii, H., Hamada, H., Kikuchi, Y.and Fujii-Kuriyama, Y.: "Two new members of the murine Sim gene family are transcriptional repressors and show different expression patterns during mouse embryogenesis." Mol.Cell.Biol.16(10). 5867-5875 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikuchi, H., Hossain, A., Ikawa.S.and Watanabe.M.: "The different inducibility of CYP1A1-mRNA between the human and the mouse cells by benzimidazole compounds." Arch.Biochem. Biophys.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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