1997 Fiscal Year Final Research Report Summary
Study of the receptor of exogastrula-inducing peptides
| Project/Area Number |
07680794
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Saitama University |
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Principal Investigator |
SUYEMITSU Takashi Saitama Univ, Fac.Sci.Dpt.Reg.Biology, Professor, 理学部, 教授 (40092019)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Exogastrula / gastrulation / Sea urchin / Exogastrula inducing peptides |
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| Research Abstract |
In order to elucidate the function of exogastrula-inducing peptides (EGIPs), EGIP-binding protein (EBP) was examined in this study. At first, EBP was purified and its N-terminal amino acid sequences were determined. The cDNA fragments of EBP were amplified by RT-PCR method using degenerate primers. Two cDNA clones, that is, EBP-alpha and EBP-beta were obtained from a cDNA library of unfertilized eggs of the sea urchin Anthocidaris crassispina using, as probe, the cDNA fragments obtained by the RT-PCR method. The base sequences of the two cDNA clones were very similar to each other (96% identity). Moreover, the two clones showed significant similarity to the extracellular matrixes of the other sea urchin embryos, that is, HLC-32 and bep4.
I examined the level of EBP-alpha mRNA during the development of sea urchin embryos. It became clear that EBP-alpha mRNA was present in unfertilized eggs. The level of the mRNA decreased gradually as development progressed to the pluteus stage, although there was a transient increase of the mRNA level at the prism stage. The recombinat EBP-alpha expressed in E.colibound to EGIP and inhibited the exogastrula-iunducing activity of EGIP.These results suggest that EBP-alpha is the binding protein of EGIP.
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