MOLECULAR ANALYSIS OF ABDOMINAL LEG DEVELOPMENT IN MUTANTS IN THE E COMPLEX OF BOMBYX MORI

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07680809
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: OKAZAKI NATIONAL RESEARCH INSTITUTES
• Principal Investigator: UENO Kohji NATIONAL INSTITUTE FOR BASIC BIOLOGY,ASSOCIATED PROFESSOR, 基礎生物学研究所, 助教授 (10143504)
• Project Period (FY): 1995 – 1996
• Keywords: HOMEOTIC GENE / MORPHOGENESIS / BOMBYX MORI

Research Abstract

The E complex is a homeotic gene complex that specifies abdominal segments in Bombyx mori. Analyzing the E^N and E^<Ca> chromosomes I have suggested that the E complex consists of the Bm Ubx, abd-A and Abd-B genes.
I am focusing to study the developmental mechanism of embryonic abdominal legs. Since I elucidated that the Bm abd-A region is deleted in the E^<Ca> chromosome and homozygous E^<Ca>/E^<Ca> embryos do not form abdominal legs, I speculate that the Bm abd-A gene is responsible for the development of abdominal legs.
I have isolated each cDNAs of the Bm Ubx, abd-A and Abd-B genes and prepared antibodies against the Bm Ubx, abd-A and Abd-B gene products. I have analyzed expression of Bm Ubx, abd-A and Abd-B gene products in embryo homozygous for E^<Kp> (Kp supernumerary legs), which reveals supernumerary abdominal legs on the abdminal second (A2) segment. I found that much amount of the Bm abd-A proteins were detected in A2 segments in the embryos homozygous for E^<Kp>. Therefore I speculated that the abnormal expression of the Bm abd-A proteins might cause development of extra abdminal legs on the A2 segment.

Research Products

• [Publications] Nagata et al: "Developmental expression of the Bondyx Autennapedia homologue and liaueotuc changes in the Nc mutant" Genes to cells. 1. 555-568 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ueno and Suzuki: "p260/270 expressed in enbryonic abdomical leg cells of Bowbyx morc can trans fer polmitate to peptide" J.Biol.Chem.(in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagata, T., Suzuki, Y., Ueno, K., Kokubo, H., Xu, X., Hui, C.-c.Hara, W.and Fukuta, M.: "Developmental expression of the Bombyx Antennapedia homologue and homeotic changes in the Nc mutant" Genes to Cells. 1. 555-568 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ueno, K.and Suzuki, Y.: "p260/270 expressed in embryonic abdominal leg cells of Bombyx mori can transfer palmitate to peptides" J.Biol.Chem.(in press).
  • Description: 「研究成果報告書概要(欧文)」より