1996 Fiscal Year Final Research Report Summary
Immunohistochemical studies on the localization of Interleukin-1 and its receptor in the hippocampal CA-1 region of the ischemic brain.
| Project/Area Number |
07680827
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
MAEDA Mitsuyo Osaka City Univ., Med.Sch., Lectu., 医学部, 講師 (40122080)
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| Co-Investigator(Kenkyū-buntansha) |
YONEDA Takunari Osaka City Univ.Med.Sch.Res.Assoc., 医学部, 助手 (70271179)
SATO Makoto Osaka City Univ.Med.Sch.Assoc.Prof., 医学部, 助教授 (10222019)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Interleukin-1 / Interleukin-1 receptor / Immunohistochemistry / brain ischemia |
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| Research Abstract |
The localization of Interleukin-1 (IL-1alpha, IL-1beta) and receptors (IL-1RtI,IL-1RtII) was immunohistochemically examined in the hippocampal CA-1 region by a transient occlusion of bilateral carotid arteries in Mongolian gerbils. Immunoreactivities for both types of IL-1 (alpha or beta) were found in reactive astrocytes, microglias and endothelial cells of capillaries in the stratum radiatum from 3 days to 30 days after the recirculation. Immunopositive reactive astrocytes with edematous changes increased in number until 30 days after the recirculation. At the electron microscopic level immunoreactive endoproducts were diffusely distributed in the cytopolasm of astrocytes, microglias and endothelial cells. Immunoreactivities for both types of IL-1R (I,II) were observed in reactive astrocytes, microglias and endothelial cells of capillaries increase in number until 30 days and also pyramidal neurons from 3 to 7 days after recirculation. Contrary to the diffuse localization of endothelial cells, immunoreactive products of IL-1R of reactive astrocytes were dispersed in the cytoplasm, especially close to the cytoplasmic membrane and apical processes around capillaries. Immunopositive pyramidal neurons showed moderate to severe morphological alterations. These findings suggest that the IL-1 system plays autocrine/paracrine role in brain ischemic insult.
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