1996 Fiscal Year Final Research Report Summary
Gene activation of CREB by light/dark cycle in the rat retina
| Project/Area Number |
07680831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
IMAKI Junko Nippon Medical School, Medical Department, Assistant professor, 医学部, 講師 (20223323)
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| Project Period (FY) |
1995 – 1996
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| Keywords | retina / CREB / in situ hybridization / c-fos mRNA / immunocytochemistry / phosphorylation / rat / Ca^<2+> |
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| Research Abstract |
The signal pathway of the light-induced gene expression in retinal cells was investigated. Flashing light induced c-fos and somatostatin in the cells of the inner border of the inner nuclear layr (INL) and the ganglion nuclear layr (GNL). As both c-fos and somatostatin genes have cAMP responsive element (CRE) in their promoters, CREB phosphorylation in retinal cells was examined with phospho-CREB specific antibody. One min exposure to flashing light induced maximum phosphorylation of CREB showing close correlation with c-fos and somatostatin mRNA expression in the same portion of the retina. Administration of L-type Ca^<2+> channel activator Bay K 8644 also induced CREB phosphorylation and c-fos and somatostatin expression in the rat retina. The distribution of calmodulin kinase II immunoreactive retina cells was in good accordance with the phospho-CREB immunoreactivity. Thus we propose that transcriptional response to light stimulation is regulated via CREB phosphorylation catalyzed by Ca^<2+>/calmodulin-dependent protein kinase in neural retina.
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[Publications] Hagiwara, M., Shimomura, A., Yoshida, K.and Imaki, J.: "Gene expression and GREB phosphorylation induced by cAMP and Ca2+ in neuronal cells.In H.Hidaka and A.C.Nairn (Ed.)" Intracellular Signal Transduction, Academic Press Inc, 525B Street, Suite 1900, San Diego. CA 92101-4495. 277-285 (1996)
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[Publications] Harada, T., Imaki, J., Ohki, K., Ono, K., Ohashi, T., Matsud, H.and Yoshida, K.: "Cone-associated c-fos gene expression in the light-damaged rat retina" Invest Ophthalmol Visual Sci. 27. 1250-1255 (1996)
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[Publications] Ohki, K., Yoshida, K., Harada, T., Takamura, M., Matsuda, H.and Imaki, J.: "c-fos gene expression in postnatal rat retinas with light/dark cycle" Vision Res. 36. 1883-1886 (1996)
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[Publications] Ohki, K., Yoshida, K., Hagiwara, M., Harada, T., Takamura, M., Ohashi, T., Matsuda, H.and Imaki, J.: "Nitric oxide induces c-fos gene expression via cyclic AMP response element binding protein (CREB) phosphorylation in rat retinal pigment epithelium" Brain Research. 696. 140-144 (1995)
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[Publications] Ohki, K., Yoshida, K., Yamakawa, A., Harada, T., Matsuda, H.and Imaki, J.: "jun-B gene expression in rat retinal cells following focal retinal injury" Current Eye Research. 14. 1021-1024 (1995)
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[Publications] Yoshida, K., Imaki, J., Fujisawa, H., Harada, T., Ohki, K., Matsuda, H.and Hagiwara, M.: "Defferential distribution of CaM kinases and induction of c-fos expression by flashing and sustained light in rat retinal cells" Investigative Ophthalmology & Visual Science. 37. 174-179 (1996)
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