1996 Fiscal Year Final Research Report Summary
Mclecular architecfure of postsynaphc density
| Project/Area Number |
07680851
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | SHINSHU UNIVERSITY |
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Principal Investigator |
SUZUKI Tatsuo Shinshu University School of Medicine Professor, 医学部, 教授 (80162965)
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| Project Period (FY) |
1995 – 1996
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| Keywords | postsynaptic densty / synaptic plasticity / signal transduction |
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| Research Abstract |
I surveyed unidentified molecules which are localized in the postsynaptic denisty(PSD).
1. Messengers from synapse to the nucleus (MSNs) : Based on the candiates listed in my review (Neurosci. Res., 25 : 1-6, 1996), linvestigated the intracellular localization of four kinds of proteins : NF-kB/lkB, cAMP-responsing element binding protein(CREB), heat shock protein(HSP)70, and MAP kinase cascade components. (1) immunoreactivities to both NF-kB and lkB were localized in PSD in vivo. (2) CREB, though only a little amount, occured in neuronal dendrites. The dendritic CREB was associated with cytoskeleton and could be released after phosphorylation by Ca^<2+>/calmodulin-dependent protein kinase. (3) HSP 70 was also localised in dendrites and especially in the synaptic region. (4) MAP kinase and its substrate RSK were found in the PSD fraction prepared from rat brain. Both proteins are known to translocate from cytoplasm to the nucleus upon certain extracellular stimuli. I am now examining the precise lacalization of the proteins at an electron microscopic level and am trying to visualize the movement of the proteins to the nucleus.
2. I am now cloning the PSD proteins using specific antibody towards PSD.I obtained one cDNA clone whose sequence has no homology with those reported previously. I will continue the cloning of the cDNA and those coding other unknown PSD proteins.
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