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1996 Fiscal Year Final Research Report Summary

Distribution of neurotrophin-3 in various regions of the brainand selected peripheraltissues and its developmental changes

Research Project

Project/Area Number 07680865
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionInstitute for Developmental Research, Aichi Human Service Center

Principal Investigator

KATOH-SEMBA Ritsuko  Department of Perinatology, Institute for Developmental Research, Aichi Human Service Center, Room Chief, 周生期学部, 室長 (80100163)

Project Period (FY) 1995 – 1996
KeywordsNeurotrophin-3 / Nerve growth factor / Brainerived neurotrophic factor / Brain / Development / Rats
Research Abstract

We have established sensitive tow-site enzyme immunoassay systems for neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF), in addition to nerve growth factor, and have shown that our immunoassay systems quantify BDFN and NT-3 with their molecular mass of 13 kDa and 14 kDa, respectively. Then, weinvestigated a regional distribution of BDNF and NT-3, and developmental changes in their levels in the brain and selected tissues from rats. Appreciable levels of BDNF was found in the olfactory bulb, septum, hippocampus, striatum, cerebral cortex, cerebellum and hypothalamus from rats at one month of age. By contrast, fairly high concentrations of NT-3 were found only in four regions from the same aged rats, such as the hippocampus, septum, cerebellum and olfactory bulb. The highest concentrations of BDNF and NT-3 were observed in the hippocampus (5.41 and 42.0ng/g, respectively). Highlevels of NT-3 were detected in the visceraltargets of the nodese ganglia while very low levels of BDNF was detected in the spleen, thyumus, hypophysis and liver, but not in other peripheral tissues. The concentration of BDNF increased during the first 30 postnatal days in all brain regions. Levels of NT-3 incressed in the olfactory bulb, hippocampus and septum during the first 60 days of age while they decreased in the cerebelum and secebral cortex. These observations seem to support the consideration that BDNF functions in adulthood and NT-3 does so in embryonic and neonatal periods. Immunocytochemical analyzes showed that NT-3 was lozalized in the CA-2-CA4 regions of teh pyramidal cell layr and BDNF was localized in the nucleus of some granule cells in the dentate gyrus and of a few neurons in the pyramidal cell layr and in the hypothalamus.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kanda et al.,: "Effect of nerve growth factor and folskolin on glycosyl transferase activities and expression of a globo-series glycosphingolipid in PC12D pheochromocytoma cells." Journal of Neurochemistry. 64. 810-817 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katoh-Semba et al.: "Chondroitin sulfate proteoglycans in the rat brain : candidates for axon barriers of sensory neurons and possible modification by laminin of their actions." European Journal of Neuroscience. 7. 613-621 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katoh-Semba et al.: "Tissue distribution and immunocytochemical localization of neurotrophin-3 in the brain and peripheral tissues of rats." Journal of Neurochemistry. 66. 330-337 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuda et al.: "A possible role of the nestin protein in the developing central nervous system in rat embryos." Brain Research. 723. 177-189 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamanaka et al.: "Immunocytochemical localization of heme oxygenase-2 in the rat cerebellum." Neuroscience Research. 24. 403-407 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 仙波りつ子他: "ニューロトロフィンの脳における加齢変化と老化脳における動態" 生体の科学. 47. 594-600 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katoh-Semba et al.: "The distribution of brain-derived neurotrophic factor in rats and its changes with development in the brain." Journal of Neurochemistry. (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanda, T., Ariga, T., yamawaki M., Paul, S., Katoh-Semba, R., Yu, R.K.: "Effect of nerve growth factor and forskokinon glyco-syltransferase activities and expression of a globo-series glyco-sphingolipid in PC12D pheochromocytoma cells" J.Neurochem.64. 810-817 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh-Semba, R., Matsuda, M., Kato, K., Oohira, A.: "Chondroitin sulfate proteoglycans in the rat brain : candidates for axon barriers of sensory neurons and the possible modification by laminin of their actions" Eur. J., Neurosci.7. 613-621 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh-Semba, R., Kaisho, Y., Shintani, A., Nagahama, M., Kato, K.: "Tissue distribution and immunocytochemical localization of neurotrophin-3 in the brain and peripheral tissues of rats" J.Neurochem.66. 330-337 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuda, M., katoh-Semba, R., Kitani, H., Tomooka, Y.: "A possible role of the nestin protein in the developing central nervous system in rat embryos" Brain Res.723. 177-189 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamanaka M., Yamabe K., Saitoh, Y., Katoh-Semba, R., Semba, R.: "Immunocytochemical lozalization of heme oxygenasse-2 in the rat cerebellum" Neurosci. Res.24. 403-407 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh-Semba, R., Takeuchi, I.K., Senba, R., Kato K.: "The distribution of brain-derived neurotrophic factor in rats and its changes with development in the brain" J.Neurochem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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