1996 Fiscal Year Final Research Report Summary
An experimental model for in vivo distant metastasis of human xenografts subcutaneously transplanted in imuune-deficient mice
| Project/Area Number |
07680921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
UEYAMA Yoshito Tokai University School of Medicine Associate Professor, 医学部, 助教授 (30072408)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Hitoshi Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (20191273)
NAKAMURA Masato Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (00164335)
TAMAOKI Norikazu Tokai University, School of Medicine, Professor, 医学部, 教授 (50055860)
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| Project Period (FY) |
1995 – 1996
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| Keywords | immune deficient mouse / lung cancer xenograft / metastasis / nm23 gene |
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| Research Abstract |
In this study, we established an experimental model for in vivo distant metastasis of human xenografts in severe combined immune deficient (SCID) mice. We examined non-metastatic (NM23) gene expression levels in the total 50 of various kinds of human tumor xenograts. We isolated human lung cancer xenografts LC-11JCK and LC-17JCK.The LC-17JCK did not express NM23 gene transcripts, whereas the LC-11JCK showed remarkable expression of NM23 gene. The xenogarafts were subcutaneously transplanted into the SCID mice. The mice were autopsied after transplantation to detect distant metastases into the lung or liver. We also examined the expression levels of vascular endothelial growth factor.
The human lung cancer xenograft LC-17JCK showed remarkable liver and lung metastases 3 months after transplantation. However, the LC-11JCK did not show any distant metastasis in the mice. We also examined the metastatic incidence of the LC-17 in the SCID/beig mice. The distant metastasis is significantly increased in the SCID/beige mice. The function of natural killer cells are additionally suppressed in the SCID/beige mice. The LC-17 xenografts also showed an increased level of VEGF gene transcripts.
These results suggest the expression levels of NM-23 gene and VEGF gene may related to distant metastases of the human lung cancer xenografts in vivo. This experimental model using severe combined immune deficient mice are potent system for the research of cancer metastasis.
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[Publications] Eiichiro Ota, Yoshiyuki Abe, Yoshiro Oshika, Yuichi Ozeki, Masayuki Iwasaki, Hiroshi Inoue, Hitoshi Yamazaki, Seiichi Tamai, Yoshito Ueyama, K.Takagi, Toshiro Ogata, Norikazu Tamaoki, Massato Nakamura: "Expression of the multidrug resistance-associated protein (MRP) gene in non-small cell lung cancer" British J.Cancer. 72. 550-554 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshiyuki Abe, Hitoshi Yamazaki, Yoshiro Oshika, Ryuji Suto, Atsushi Tsugu, Eiichiro Ota, Hidesuke Satoh, Yasuyuki Ohnishi, Tadashi Yanagawa, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura: "Advantage of in vivo chemosensitivity assay to detect vincristine-resistance in a human epidermoid carcinoma xenografts" Anticncer Research. 16. 729-734 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshiyuki Abe, Yasuyuki Ohnishi, Masumi Yoshimura, Eiichiro Ota, Yuuichi Ozeki, Yoshiro Oshika, Tetuji Tokunaga, Hitoshi Yamazaki, Yoshito Ueyama, Toshiro Ogata, Norikazu Tamaoki, Masato Nakamura: "P-glycoprotein-mediated multidrug resistance of human lung cancer cell in vivo" British J.Cancer. 74. 1929-1934 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshiyuki Abe, Yoshiro Oshika, Yasuyuki Ohnishi, Ryuji Suto, Tetsuji Tokunaga, Hitoshi Yamazaki, Hiroshi Kijima, Nobuyoshi Hiraoka, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura: "A xenograft line of human teratocarcinoma established by serial transplantation in severe combined immunodeficient (SCID) mice" APMIS. 105 (in press). (1997)
Description
「研究成果報告書概要(欧文)」より
