1996 Fiscal Year Final Research Report Summary
Tissue specific gene expression driven by CD34 promoter in transgenic mice.
| Project/Area Number |
07680922
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | TOKAI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
ANDO Kiyoshi TOKAI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (70176014)
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| Project Period (FY) |
1995 – 1996
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| Keywords | CD34 / HEMATOPOIETIC STEM CELL / TRANSGENIC MICE |
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| Research Abstract |
CD34 is a glycoprotein which locates on the cell surface of hematopoietic progeniter/stem cells and vascular endothelial cells. Hematopoietic stem cells pocess both self-renewal and multipotent ability. They reconstitute hematopoietic and immune system of the host after bone marrow transplantation. They are also assumed as ideal targets of gene transfer in gene therapy. In spite of the clinical importance mentioned above, the mechanism of growth control in these hematopoietic stem cells remains unknown because they are extremely rare population (frequency of 10^<-5>) in bone marrow cells and are difficult to be manupulated in vitro. To overcome these difficulties we attempted introduction of CD34 genomic DNA into mouse fertilized eggs so that its tissuse specific expression would be attained in vivo. We microinjected genomic DNA of human CD34 containing 5 kb of 5' non-coding region, cDNA and 4 kb of 3' non-coding region into mouse fertilized eggs. Two out of 17 mice we generated were confirmed to have transgenes. So far expression of human CD34 was not detected in any tissue obtained from F1 progeny of these transgenic mice.
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