1996 Fiscal Year Final Research Report Summary
Study on Biomaterials for Drug Delivery Sysytem Based on Dynamic Structural Change of Molecular Assembly of Elastin Peptides
| Project/Area Number |
07680946
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
OKAMOTO Kouji Kyushu Institute of Technology, Department of Biochemical Engineering and Science, Professor, 情報工学部, 教授 (40122618)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Yuko (山下 祐子) Kyushu Institute of Technology, Department of Biochemical Engineering and Scienc, 情報工学部, 助手 (80203488)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Elastin / Molecular assembly / Dynamic structural change / Drug delivery system |
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| Research Abstract |
<1995>A high polymer based on the repeating pentapeptide sequence Val-Pro-Gly-Val-Gly which has the property of salf-assembly, a peptide Arg-Gly-Tyr-Ser-Leu-Gly which can be phosphorylated by protein kinase, and two peptides Gly-Ser-Asn-His-Gly and Gly-Arg-Asn-Thr-Gly in which Asn residue can be converted to Asp residue, were synthesized. Arg-Gly-Tyr-Ser-Leu-Gly was phosphorylated by cyclic AMP dpendent protein kinase. The half-lives for Asn-Asp conversion were 6 days and 28 days for Gly-Ser-Asn-His-Gly and Gly-Arg-Asn-Thr-Gly.
<1996>Poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser-Leu-Gly)] which consists of the indicated molar ratios was synthesized. This polymer was phosphorylated by cyclic AMP dependent protein kinase for 24 hr at 30ﾟC.The resulting phosphorylated polymer poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser (PO_4^<2->) -Leu-Gly)] was studied for self-assembly. The temperature for the inverse temperature transition (Tm) of the phosphorylated polymer was shifted to higher temperature giving a shift of 14ﾟC.The phosphorylated polymer was incubated with alkaline phosphatase for 4 hr at 30ﾟC to yield a dephosphorylated poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser-Leu-Gly)]. Tm was shifted to lower temperature giving a shift of 13ﾟC.This result indicated complete dephosphorylation of phosphorylated polymer.
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