1995 Fiscal Year Annual Research Report

下垂体腺腫の細胞起源と遺伝子異常に関する研究

Research Project

Project/Area Number	07771082
Research Institution	Teikyo University
Principal Investigator	松野彰帝京大学, 医学部, 講師 (00242058)
Keywords	下垂体腺腫 / MEN-1遺伝子 / Gsα遺伝子 / 内分泌能
Research Abstract	凍結保存してあった10例の下垂体腺腫組織(成長ホルモン産生腺腫5例、プロラクチン産生腺腫5例)より、proteinase K-phenol-chloroform法を用いてDNAを抽出した。今回はMEN-1の遺伝子のallelic deletionの有無を検討し、成長ホルモン産生腺腫についてはGsα遺伝子のmutationの有無を検討した。MEN-1とGsα遺伝子の遺伝子の特定の部位をpolymerase chain reactionにより増幅し、増幅された遺伝子産物をpolyacrylamide gelで電気泳動し、single strand conformation polymarphism(SSCP)法により解析し、allelic deletionの有無を検討した。同時に免疫組織化学染色やin situ hybridization法により、下垂体腺腫の内分泌能についても検討した。今回の検討では、いずれの腺腫においてもこれらの遺伝子の変異は認められなかった。従ってその内分泌能との相関については明らかな知見は得られなかった。今後はGH、PRL、TSHの転写因子として働くPit-1の発現を免疫組織化学染色やin situ hybridization法により検討し、個々の腺腫の内分泌能との関連について調べたいと考えている。

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Matsuno A et al.: "Changes in the ultrastructural distribution of prolactin and growth hormone mRNAs in pituitary cells of female rats after estrogen and bromocriptine treatment, studied using in situ hybridization with biotinylated oligonucleotide probes" Histochem Cell Biol. 104. 37-45 (1995)
[Publications] Matsuno A et al.: "Transcavemous surgery; an effective treatment for pituitary macroadenomas" Eua J Endocrinol. 133. 156-165 (1995)
[Publications] Sanno N et al.: "GH and PRL gene expression by nonradioisotopic in situ hybridization in TSH-secreting pituitary adenomas" J Clin Endocr Metab. 80. 2518-2522 (1995)
[Publications] Matsuno A et al.: "Clinical an histopathological analysis on proliferative potentials of recurrent and nonrecurrent meningiomas" Acta Neuropathol. (in press).
[Publications] Itoh J et al.: "An application of confocal laser scanning microscopy (CLSM) to visualize immunohistochemical localization of prolactin (PRL) and in situ hybridization of prolactin (PRL) mRNA in the rat pituitary gland, without using fluorescent probes" Microscopy Research and Techiqve. (in press).
[Publications] Sanno N et al.: "Expression of human Pit-1 product in the human pituitary and pituitary adenomas" Arch Pathol Lab Med. (in press).
[Publications] Sanno N et al.: "Combined non-isotopic in situ hybridization method and indirect immunohistochemical analysis of hormone production in rat pituitary" J Clin Mol Pathol. (in press).
[Publications] Sanno N et al.: "In situ hybridization analysis of Pit-1 mRNA and hormonal produciton in human pituitary adenomas" Acta Newopathol. (in press).
[Publications] Sanno N et al.: "Pit-1 and estrogen receptor mRNA expression in prolactinomas" Modern Pathd. (in press).
[Publications] 松野彰ら: "非放射性プローブを用いたin situ hybridization法による下垂体腺腫の下垂体前葉ホルモンmRNA発現の検討およびその電顕レベルへの応用" ホルモンと臨床. 43. 94-98 (1995)
[Publications] 松野彰ら: "免疫組織化学における分子遺伝学の役割電顕レベルのin situ hybridization法" 臨床検査免疫組織細胞化学検査. 39. 17- (1995)

1995 Fiscal Year Annual Research Report

下垂体腺腫の細胞起源と遺伝子異常に関する研究

Principal Investigator

松野 彰 帝京大学, 医学部, 講師 (00242058)

Research Products

[Publications] Matsuno A et al.: "Transcavemous surgery; an effective treatment for pituitary macroadenomas" Eua J Endocrinol. 133. 156-165 (1995)

[Publications] Sanno N et al.: "GH and PRL gene expression by nonradioisotopic in situ hybridization in TSH-secreting pituitary adenomas" J Clin Endocr Metab. 80. 2518-2522 (1995)

[Publications] Matsuno A et al.: "Clinical an histopathological analysis on proliferative potentials of recurrent and nonrecurrent meningiomas" Acta Neuropathol. (in press).

[Publications] Sanno N et al.: "Expression of human Pit-1 product in the human pituitary and pituitary adenomas" Arch Pathol Lab Med. (in press).

[Publications] Sanno N et al.: "Combined non-isotopic in situ hybridization method and indirect immunohistochemical analysis of hormone production in rat pituitary" J Clin Mol Pathol. (in press).

[Publications] Sanno N et al.: "In situ hybridization analysis of Pit-1 mRNA and hormonal produciton in human pituitary adenomas" Acta Newopathol. (in press).

[Publications] Sanno N et al.: "Pit-1 and estrogen receptor mRNA expression in prolactinomas" Modern Pathd. (in press).

[Publications] 松野彰ら: "非放射性プローブを用いたin situ hybridization法による下垂体腺腫の下垂体前葉ホルモンmRNA発現の検討およびその電顕レベルへの応用" ホルモンと臨床. 43. 94-98 (1995)

[Publications] 松野彰ら: "免疫組織化学における分子遺伝学の役割 電顕レベルのin situ hybridization法" 臨床検査 免疫組織細胞化学検査. 39. 17- (1995)

松野彰帝京大学, 医学部, 講師 (00242058)

[Publications] 松野彰ら: "免疫組織化学における分子遺伝学の役割電顕レベルのin situ hybridization法" 臨床検査免疫組織細胞化学検査. 39. 17- (1995)