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1997 Fiscal Year Final Research Report Summary

Relationship between Cytosolic Acetyl-CoA Hydrolase Activity and Pathophysiological states of rats

Research Project

Project/Area Number 07807018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

ISOHASHI Fumihide  St. Marianna University School of Medicine, Deparment of Biochemistry, Professor, 医学部, 教授 (20115992)

Project Period (FY) 1995 – 1997
KeywordsAcetyl-CoA-Hydrolase / Diabetes / Cholesterol / Hepatocarcinogenesis / Rat liver / Enzyme purification / CoA / 3'-Methyl-4-dimethylaminoazobenzene
Research Abstract

The activity of cytosolic acetyl-CoA hydrolase increased in the early diabetic, cholesterol-fed, a potent competitive inhibitor of microsomal 3-hydroxy-3-methylglutaryl-CoA reductase (CS-514), and a hypolipidemic drug [alpha- (rho-chlophenoxy) isobutyric acid, CPIB) treated groups. The cholestyramine treatment of cholesterol-fed rats made the enzyme activity return to the initial level. In chronic diabetes, the enzyme activity was within normal range but increased significantly when given the cholesterol-diet, CPIB,or CS-514. An increase in the enzyme activity by these treatments was associated with an increase in enzyme protein determined by enzyme-linked immunosorbent assay. These resuls suggest that this enzyme has a physiological role in the maintenance of the equilibrium between the cytosolic acetyl-CoA concentration and CoASH pool for cholesterol metabolism.
When Donryu male albino rats were given diet containing 0.06% 3'-methyl-4-dimethyl-aminoazobenzene (3'-Me-DAB) for 20 weeks, the activity of cytosolic acetyl-CoA hydrolase in their livers decreased to about one-third the initial level in week 2, returned to the control level in week 7, and then decreased again to anout one-tenth of the control in week 20. These changes in enzyme activity were parallel with changes in the amount of enzyme protein determined by ELISA.In 3'-Me-DAB-resistant rats, however, the enzyme activity and enzyme protein remained within the normal range during administration of 3'-Me-DAB-containing diet for 20 weeks and no tumors were detectable macroscopically. Interestingly, the biphasic change in this enzyme activity was inversely associated with the well known change of gamma-glutamyltranspeptidase activity during azo-dye-induced hepatocarcinogenesis.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Okamoto, K.: "Effect of Macromolecular-Translocation Inhibitor-III on Binding of Activated Glucocorticid-Receptor Complex to Specific DNA" J.Biochem.119. 920-925 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suematsu, N.: "Evaluation of Various Purification Methods for Fluorescent Extension Products of DNA Sequencing Reaction" St.Marianna Med.J.24. 557-565 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suematsu, N.: "Effects of Various Proteins or Peptides on Reactivation of Cold-Inactivated Acetyl-CoA Hydrolase from Rat Liver" St.Marianna Med.J.24. 691-697 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shibata, K.: "Cloning of Nuclear Receptor Superfamily Glucocorticoid Receptor cDNA with Polymerase Chain Reaction" 石巻専修大学 研究紀要. 59-64 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末松 直也: "低温感受性アセチルCoA水解酵素のインスリンによる不活性化" ビタミン. 71. 87-88 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 礒橋 文秀: "ラット肝細胞質アセチルCoA水解酵素の低温失活状態よりのサーファクタントによる活性回復促進効果" ビタミン. 72. 65 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正夫: "平成6年度報告書" 高齢者社会福祉研究第5号川崎市社会福祉総合センター, 32 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村正夫: "平成7年度報告書" 高齢者社会福祉研究第6号川崎市社会福祉総合センター, 14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamoto, K., and Isohashi, F.: "Effect of Macromolecular-Translocation Inhibitor-III on Binding of Activated Glucocorticoid-Receptor Complex to Specific DNA" J.Biochem.119. 920-925 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suematsu, N., Okamoto, K., and Isohashi, F.: "Evaluation of Various Purification Methods for Fluorescent Extension Products of DNA Sequencing Reaction." St.Marianna Med.J.24. 557-565 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suematsu, N., Okamoto, K., and Isohashi, F.: "Effects of Various Proteins or Peptides on Reactivation of Cold-Inactivated Acetyl-CoA Hydrolase from Rat Liver." St.Marianna Med.J.24. 691-697 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shibata, K., Okamoto, K., and Isohashi, F.: "Cloning of Nuclear Receptor Superfamily Glucocorticoid Receptor cDNA with Polymerase Chain Reaction" Journal of Ishinomaki Senshu University. 8. 59-64 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, K., and Isohashi, F.: "Purification and Characterization of Rat Hepatic Macromoleculer-Translocation Inhibitor-III of Activated Glucocorticoid-Receptor Binding to Nuclei and Specific DNA." Proceedings of 12th International Symposium of Federation of Asian and Oceanian Biochemists and Molecular Biolagists. 133 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isohashi, F., and Okamoto, K.: "Interference of Macromorecular-Translocation Inhibitor-III in the Binding of Activated Glucocorticoid-Receptor Complex to Specific DNA." Proceedings of 17th International Congress of Biochemistry and Molecular Biology. 923 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suematsu, N., and Isohashi, F.: "Enhancement of reactivation of cold-inactivated acetyl-CoA hydrolase from rat liver cytosol with Triton 100-X." Proceedings of 25th anniversary FAOBMB Symposium, Bulletin of the Philippine Society for Biochemistry and Molecular Biology. 16. 62 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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