1996 Fiscal Year Final Research Report Summary
Studies for pathogenesis of erythema migrans caused by Lyme disease Borrelia
| Project/Area Number |
07807032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
ISOGAI Emiko Health Sciences University of Hokkaido, Department of Preventire Dentistry, Instructor, 歯学部, 助手 (80113570)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Lyme disease / Borrelia burgdorferi / erythema migrans / platelet-activating-factor / inflammatory cytokines / platelets / neutrophils / endothelial cells |
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| Research Abstract |
This study describes a role of platelet-activating factor (PAF) as a potential inducer of inflammation in infection with Borrelia burgdorferi. Following approaches were done.
1) The first involved the use of a PAF antagonist to show the lack of an inflammatory response in skin lesions.
2) The second was to show that the PAF antagonist reduced platelet aggregation when the spirochetes were incubated with polymorphonuclear leukocytes (PMN).
3) The third was to show that the PMN,which were activated by the spirochetes, inhibited collagen-platelet aggregation.
3) The forth was to show the effect of spirochetes on the adherence of platelets and PMN to cultured human umbilical vein endothelial cells (HUVEC). The PAF antagonist reduced the cell-adherence to HUVEC.
These observations may contribute to a better underestanding of the pathogenesis of Lyme disease spirochetes in the local inflammatory reaction.
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Research Products
(28 results)
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[Publications] Kimura, K., Isogai, E., Isogai, H., Nishikawa, T., Masuzawa, T., Yoshikawa, K., Kotake, S., Ohno, S., and Fujii, N.: "Prevalence of antibodies against Borrelia species in patients with unclassified uveitis in regions in which Lyme disease is endemic and nonendemic." Clin.Diag.Labo.Immunol.2. 53-56 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kimura, K., Isogai, E., Isogai, H., Kamewaka, Y., Nishikawa, T., Ishii, N., and Fujii, N.: "Detection of lyme disease spirochetes in the skin of naturally infected wild sika deer (Cervus nippon yesoensis) by PCR." Appl.Environment.Microbiol.61. 1641-1642 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, N., Isogai, E., Isogai, H., Kimura, K., Nishikawa, T., Fujii, N., and Nakajima, H.: "T cell response to Borrelia garinii, Borrelia afzelii, and Borrelia japonica in various congenic mouse strains." Microbiol.Immunol.39. 929-935 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Isogai, E., Kimura, K., Fujii, VN., Nishikawa, T., Ishii, N., Postic, D., Baranton, G., and Isogai, H.: "Platelet-activating-factor-mediated pathogenesis in Lyme disease." Infect.Immun.64. 1026-1029 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Isogai, E., Isogai, H., Masuzawa, T., Postic, D., Baranton, G., Kamewaka, Y., Kimura, K., Nishikawa, T., Fujii, N., Ishii, N., Ohno, S., and Yamaguchi, N.: "Borrelia burgdorferi sensu lato in an endemic environment : wild sika deer (Ceruvus nippon yesoensis) with infected ticks and antibodies." Micorbiol.Immunol.40. 13-19 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Masuzawa, T., Wilske, B., Komikado, T., Suzuki, H., Kawabata, H., Sato, N., Muramatsu, K., Sato, N., Isogai, E., Isogai, H., Johnson, R.C., and Yanagihara, Y.: "Comparioson of ospA serotypes for Borrelia burgdorferi sensu lato from Japan, Europe and North America" Micorbiol.Immunol.40. 539-545 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Yukikake, M., Kuroda, Y., Kurohara, K., Matsui, M., Yasuda, A., Matsui, Y., Oono, S., Kimura, K., and Isogai, E.: "Occurrence of Borrelia garinii oculo-neuroborreliosis in Japan." J.Neuro.Sci.142. 160-161 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Isogai, E., Isogai, H., Kimura, K., Hayashi, S., Kubota, T., Nishikawa, T., Nakane, A., and Fujii, N.: "Cytokines in the serum and brain in mice infected with distinct species of Lyme disease Borrelia." Microbial Pathogenesis. 21. 413-419 (1996)
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