1997 Fiscal Year Final Research Report Summary
Tissue engineering by hepatocyte transplantation as an artificial support for the liver function
| Project/Area Number |
07807116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
TAKEDA Taichi Kyorin University School of Medicine, Instructor, 医学部, 助手 (20271249)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Tissue Engineering / Artificial Liver / Hepatocyte Transplantation |
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| Research Abstract |
In situ collagenase perfusion technique was established to successfully isolate rat hepatocytes.
In order to achieve transplantation of the hepatocytes using a method of tissue engineering, the cells were cultivated in a heat-treated collagen sponge in rat. However, the implanted spones between mesenteries induced granulation of the tissue without adequate vascularization. Since collagen sponge was easily compressed with the granuIation, nylon mesh was used to reinforce the structure, but it did not enhance the vascularization in the implanted sponges.
Hepatocytes in a sponge degenerated in a week in vivo. Histological examinations of the tissue revealed apoptosis and inflammatory reactions. Fucidine was systemically given to the recipient to suppress neutrophil function, which did not show any beneficial effectto maintain the implanted hepatocytes, nor did ascorbic acid used as an antioxidant. Further studies on hepatatrophic stimulants will be required to promote hepatocyte transplantation as an artificial support for the liver function.
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