開心術後肺虚血再濯流障害に対する一酸化窒素(NO)吸入療法の確立

1995 Fiscal Year Annual Research Report

• Project/Area Number: 07807127
• Research Institution: Toyama Medical and Pharmaceutical University
• Principal Investigator: 村上 新 富山医科薬科大学, 附属病院, 助手 (70190874)
• Co-Investigator(Kenkyū-buntansha): 深原 一晃 富山医科薬科大学, 附属病院, 医員 ; 市田 蕗子 富山医科薬科大学, 附属病院, 講師 (30223100)
• Keywords: pulmonary hypertension / ischemia-reperfused lung / superoxide dismutase / lipid peroxide / pulmonary vascular resistance / NO inhalation

Research Abstract

Method; Fourteen anesthetized mongrel dogs were mechanically ventilated. After 90 min. of the left lung ischemia by the left hilum clamping, the same side lung reperfusion for 150 min. was achieved by declamping of the left hilum and clamping of the opposite side hilum so that all the perfusion and ventilation was supported only by the left lung. Seven dogs inhaled 30ppm NO during reperfusion (Group 1). The other seven dogs were ventilated without NO (Group 2). Hemodynamic parameters, blood gas, superoxide dismutase(SOD)activity and lipid peroxide content in the left atrial blood and the left lung wet/dry ratio at the end of the experiment were analysed.
Results; Immediately after reperfusion, systolic pulmonary arterial pressure was significantly lower in Group 1(27.6±4.5mmHg vs 44.3±6.3mmHg,p<0.05).This significant reduction of The pulmonary blood pressure continued throughout reperfusion. Significant improvement was also observed in Group 1 on pulmonary vascular resistance and right ventricular enddiastolic pressure. SOD activity 15 min. after reperfusion in Group 2 droped to 41% of pre-ischemic value. On the other hand, in Group 1, any significant decrease was not observed during reperfusion. Left lung wet / dry ratio was significantly lower in Group 1(4.72±0.36 vs 5.47±0.52,p<0.05).

Research Products

• [Publications] 村上 新: "肺高血圧モデルを用いた一酸化窒素吸入の実験的検討" 日本小児循環器学会雑誌. 11. 268 (1995)
• [Publications] 深原一晃: "肺高血圧を伴う先天性心疾患における各種血管拡張剤の投与による術前評価の意義" 日本胸部外科学会雑誌. 43. 1496 (1995)
• [Publications] 深原一晃: "肺虚血再濯流後の一酸化窒素(NO)吸入の効果とフリーラジカル済去能に関する検討" 日本循環器学会誌(Suppl I). 59. 391 (1995)