Molecular cloning and clinical application of the endometriosis-specific genes

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07807156
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: OSAKA CITY UNIVERSITY
• Principal Investigator: OGITA Sachio Osaka City University, Medical School, Professor, 医学部, 教授 (00047086)
• Co-Investigator(Kenkyū-buntansha): UMESAKI Naohiko Osaka City University, Medical School, Associate Professor, 医学部, 助教授 (20106339)
• Project Period (FY): 1995 – 1996
• Keywords: Endometriosis / cDNA cloning / endometrium / differential display

Research Abstract

To complete this study successfully, the following two hypotheses should have been reproducible : First, distinct difference in mRNA expressions between endometriotic eutopic endometrial tissues and non-endometriotic eutopic endometrial tissues ; Second, high reproducibility in cDNA cloning by the method of differential display. However we found unpredicted problems by which we had to change the direction of this study. The problems were as follows : Although there found certain differences in biological activities of eutopic endometrial stromal cells between endometriotic patients and non-endometriotic women, we could not find any stable genetic differences in them as far as we investigated. Possible causes of the unstable differences indicate that clinically healthy women can have minimal endometriotic lesions in them, and that endometriosis may cause secondary stimuli which can affect abnormal gene expressions in eutopic endometriotic stromal cells. As for differential display, we a pplied the original method by using random 12mers according to the original procedure. But this method, as described before in our previous intermediate report on this study, happened to amplify a minor contaminated cDNAs and/or to isolate an unreproducible cDNA.Few successful report in cDNA cloning by differential display has been published yet in fact, this method was thought to be exciting approach of cDNA cloning when the method was published. Accordingly we have tried some modifications in differential display in vain. Since we have found some technical problems in this study when we performed many experiments, the following cellular natures in endometriosis to identify and clarify the specific genetic disorders to endometriosis. Recently we have disclosed that long-term in vitro cultured eutopic endometrial stromal cells show the differential expressions of some surface antigens between endometriotic patients and non-endometriotic women, and that anti-endometriotic antibodies found in endometriotic patient sera have certain tissue-specificity and specific biological activity. These results may provide some clues to clone cDNAs for endometriosis-related genes.

Research Products

• [Publications] Tanaka T.et al.: "Cytokinc regulation of Fas-mediatcd apoptotic signals in human endometrial epithelium" proceeding of 9th JSIR. 10. 21-22 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizuno K et al.: "Characterization of apoptotic signals mediated by TNF-receptor family in human endomctrium:Regulation by cytokines" proceeding of 9th JSIR. 10. 79-80 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tanaka T.et al.: "Establishmcnt and characterization of B1-derived macrophage cell line:Effects of anti-endometriotic medicine,Danazol,on the macrophage cells and the parent B1 cells." Proceeding of 11th JSIR. (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Chang L.et al.: "Regulating system in susceptibility to apoptosis in human endometrial epithelium:Regulation by specific binding of endometrial cells to extracellular matrices." proceeding of 11th JSIR. (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagashima T et al.: "Fas-mediated apoptosis in human endometrial epithelium and intrauterine cytokine network." Proceeding of 11th JSIR. (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizuno K et al.: "Flowcytometric characterization of anti-endometrial antibodics in endometriotic patient sera." Proceeding of 11th JSIR. (in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tanaka T,Mizuno K,Miyama M,Nakamura H,Mine M,Hashiguchi Y,Umesaki N,Ogita S,Yamanoto I,Umeki K,Ohtaki S.: "Cytokine regulation of Fas-mediated apoptotic signals in human endometrial epithelium." JSIR. 10. 21-22 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizuno K,Tanaka T,Miyama M,Nakamura H,Mine M,Hashiguchi Y,Umesaki N,Ogita, S,Yamamoto I,Umeki K,Ohtaki S: "Characterization of apoptotic signals mediated by TNF-receptor family in human endometrium : Regulation by cytokines." JSIR. 10. 79-80 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tanaka T,Chang L,Chen H,Miyama M,Umesaki N,Ogita S: "Establishment and characterization of B1-derived macrophage cell line : Effects of anti-endometriotic medicine, Danazol, on the macrophage cells and the parent B1 cells." JSIR. 11 (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Chang L,Tanaka T,Mizuno K,Chen H,Miyama M,Umesaki N,Ogita S: "Regulating system in susceptibility to apoptosis in human endometrial epithelium : Regulation by specific binding of endometrial cells to extracellular matrices." JSIR. 11 (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nagashima T,Tanaka T,Mizuno K,Chang L,Chen H,Miyama M,Umesaki N,Ogita S: "Fasmediated apoptosis in human endometrial epithelium and intrauterine cytokine network." JSIR. 11 (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizuno K,Tanaka T,Chen H,Miyama M,Fujino Y,Umesaki N,Ogita S: "Flowcytometric characterization of anti-endometrial antibodies in endometriotic patient sera." JSIR. 11 (in press). (1997)
  • Description: 「研究成果報告書概要(欧文)」より