1997 Fiscal Year Final Research Report Summary

Immunohistochemical studies of abnormal extrinsic nerve fibers in Hirschsprung's disease

Research Project

Project/Area Number	07807163
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	小児外科
Research Institution	Kyorin University
Principal Investigator	NIRASAWA Yuji Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (60129591)
Co-Investigator(Kenkyū-buntansha)	AKAGAWA Kimio Kyorin University, School of Medicine, Professor, 医学部, 教授 (80129303) ITO Yasuo Kyorin University, School of Medicine, Professor, 医学部, 教授 (80110881)
Project Period (FY)	1995 – 1997
Keywords	HPC-1 antigen / Hirschsprung's disease / exocytosis-related protein
Research Abstract	The HPC-1 antigen was originally identified as a neuron-specific membrane protein in the central nervous system. So, as the first step of the experiments, the presence of HPC-1 antigen in the nervous system of the rat gastrointestinal tract was immunohistochemically confirmed. The HPC-1 is thought to suppress the excess axon-collateral sprouting. Abnormal extrinsic nerve fibers in Hirschsprung's disease was then immunohistochemically examined using antibody againt the exocytosis-related protein which the HPC-1belongs to. All six exocytosis-related Proteins were succesfully demonstrated in normal sigmoid colon. On the other hand, the HPC-1 was not detected in aganglionic portion of the colon, which may explain the reason of exess extrins nerve fibers in aganglionic portion of the colon in Hirschsprung's disease.