1997 Fiscal Year Final Research Report Summary
Application to the personal identification by DNA analysis from teeth
| Project/Area Number |
07807191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
HANAOKA Yoichi TOKYO DENTAL COLLEGE,DEPARTMENT OF DENTISTRY,LECTURER, 歯学部, 講師 (30180912)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Legal Medicine / Forensic Odontology / tooth / DNA analysis / Sex determination / Personal identiofication / Amelogenirgene / D4S43 locus |
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| Research Abstract |
This research aim at the establishment of positive personal identification method from teeth, by adding DNA analysis technology to dental evidences that is useful for personal identification.
1.The sex determination using DNA obtained from tooth samples by PCR amplification of the segments of the amelogenin gene was investigated.
X-and Y-specific sequences were not detected from hard tooth tissue as degraded DNA source, in accordance with previously announced reports. However, when the size of the PCR product was shortened, X-and Y-specific sequences were completely amplified in all of the tooth semples.
2.VNTR polymorphism at the D4S43 locus was investigated using DNA from teeth. The one-repeat-unit allele (184 bp) at the D4S43 locus, which comes in four sizes (this research result on 1996), was detected in all of the degraded DNA samples obtained from hard tooth tissue. Therefore, this new size variation is useful in personal identification by DNA analysis using teeth.
These results shows that it was possible to conduct DNA analysis by PCR amplification using teeth when the size of the target DNA was small.
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