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1996 Fiscal Year Final Research Report Summary

The changes of the carbohydrate structure during neurite formation in PC12 cells and the role of poly-N-acetyllactosamine chains in the differentiation of nerve cells.

Research Project

Project/Area Number 07807205
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKYOTO SANGYO UNIVERSITY

Principal Investigator

FUKUI Shigeyuki  Dept.Biotechnology, Fac.Engineering, Kyoto Sangyo University, 工学部, 助教授 (30218888)

Co-Investigator(Kenkyū-buntansha) FUKUI Shigeyuki  Dept.Biotechnology, Fac.Engineering, Kyoto Sangyo University (30218888)
Project Period (FY) 1995 – 1996
KeywordsPC12 cells / neurite formation / poly-N-acetyllactosamine / nerve growth factor / 糖タンパク質 / 糖鎖
Research Abstract

To explore the biological role of carbohydrate chains in relation to the process of nerve cell differentiation, I have carried out comparative study to characterize the changes of carbohydrate structure of cell surface glycoproteins by using conventional PC12 cells and its variant cells (PC12D). PC12D cells extend neurites faster than PC12 cells responding not only to nerve growth factor (NGF), but also to cyclic AMP.PC12D cells differ also morphologically from PC12 cells, being flat in shape and having expended short processes without any stimulation. To know whether the carbohydrate chains of cell surface glycoproteins of PC12 cells are alterd during neurite formation, and also differ from those of PC12D cells, I used the method of in vitro metabolic labeling of those cells by using either [^3H] glucosamine to label carbohydrate moieties, or [^3H] threonine to lable peptide portion of glycoproteins. In found that the size of carbohydrate chains obtained from PC12D cells was smaller in molecular weight in general than that of PC12 cells and the difference depended particularly on the length of poly-N-acetyllactosamine chain linked to a N-linked complex oligosaccharide core. Further, the NGF stimulation caused the decreased of poly-N-acetyllactosamine synthesis from PC12 cells while PC12D cells were insensitive in this regard.
From the results of the enzyme activity of glycosyltransferases which may participate in poly-N-acetyllactosamine synthesis, the decrease in the amount of the poly-N-acetyllactosamine chains in PC12D cells and NGF-primed PC12 cells was accounted for by the lowered activity of poly-N-acetyllactosamine extension enzyme (GnT-i), for the content of poly-N-acetyllactosamine chains in PC12 and PC12D cells correlated precisely with the changes in the GnT-i activity, whereas GlcNAc transferase V (GnT-V) and beta1-4 galactosyltransferase activities remained unchanged.

  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] S.Fukui et al: "Comparison of the expression of cell surface poly-N-acetyllactosamine-type oligosaccharides in PC-12 cells with those its variant PC12D." Glycocojugate J. 12. 472-473 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Fukui et al: "The role of poly-N-acetyllactosamine chains in the differantiation of necod cells." Acta Human.Sci.Univ.Sangio kyotiensis.24. 1-76 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福井成行: "糖タンパク質の糖鎖を認識する単クローン抗体を用いた癌関連糖鎖抗原の探索と癌診断への応用" 三共生命科学研究報告集. 9. 147-158 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Fukui, M.Fukuzumi and I.Yamashina: "Comparison of the expressioin of cell surface poly-N-acetyllactosamine-type oligosaccharides in PC12 cells with those in its variant PC12D." Glycoconjugate J. 12. 472-473 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Fukui an M.Fukuzumi: "The role of poly-N-acetyllactosamine chains in the differentition of nerve cells : Comparison of the carbohydrate chains of cell surface glycoprotein in PC12 pheochromocytoma cells with those in its variant PC12D" Acta Hunanistica Et Scientifica Universitatis Sangio Kyotiensis. 24. 1-76 (1966)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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