1995 Fiscal Year Annual Research Report

発生・形態形成の個体レベルにおける分子生物学的研究

Research Project

Project/Area Number	07NP0701
Research Institution	Kyoto University
Principal Investigator	竹市雅敏京都大学, 大学院・理学研究科, 教授 (00025454)
Co-Investigator(Kenkyū-buntansha)	松居靖久東北大学, 加齢医学研究所, 助教授 (40241575) 山村研一熊本大学, 医学部, 教授 (90115197) 相沢慎一熊本大学, 医学部, 教授 (60073011) 黒岩厚名古屋大学, 理学部, 教授 (20134611) 安田國雄奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (30025473)
Keywords	発生 / 遺伝子ターゲッティング / 形態形成 / 細胞分化 / 細胞認識 / 神経発生 / 遺伝子トラッピング / ES細胞
Research Abstract	1.3種類のカドヘリンの脳におけるカドヘリンの発現パターンを解析した結果、それぞれが異なる神経回路において発現することがわかった。さらに、カドヘリン結合タンパク質カテニンがシナプス結合に局在することが確定した。これらにより、カドヘリン/カテニンが神経間の結合と回路形成に関与する可能性はますます高まった。なお、カドヘリン11の遺伝子ノックアウトに成功したので、その解析により本問題を検証中である。また、ショウジョウバエの神経系カドヘリンを2種類クローニングし、その一つ、については、突然変異体を得てその形質を解析中である。 2.FAK欠失個体の解析により、この分子がfocal adhesionのターンオーバーに関与することが示唆され、また、個体レベルではのう胚期に発生異常が観察された。 3.Otx2欠失マウスの解析を深め、ロンボメア2より前方の頭部が欠損することが明らかになり、この領域形成にとって必須であることが証明された。 4.HES1欠損マウスの解析し、Mash-1の発現が著しく増加し神経分化が正常より早く進行していることがわかった。その結果、頭部では神経管閉鎖不全、網膜では層構造の乱れがみらえた。これらの結果から、HES-1は分化の速度を抑制する役割を担っていることが明らかになった。 5.δEF-1の欠失マウスの解析し、その欠失の領域に依存して、T細胞の分化、骨格系などに異常がおきることが判明した。 6.jmj遺伝子欠損マウスの詳細な解析の結果、この遺伝子が巨核球細胞の発生に必須であることが示された。 7.肢芽形成におけるBMP-2/BMP-4の機能を解明するため、そのドミナントネガティブ分子を強制発現させた。その結果、予定細胞死領域における細胞死が抑制された。また、肢芽間充織細胞の培養にBMPを与えるとアポトーシスが誘導された。すなわち、BMPはプログラム細胞死のメディエーターであることが明にされた。 8.レセプターチロシンキナーゼSkyに関して、これが始原生殖細胞で発現し、同時にそのリガンドが生殖隆起の間質細胞で発現することにより、後者が前者の増殖を支えていることが示唆された。 9.グリア細胞特異的遺伝子repoをニューロブラストにおいて強制発現させる実験を行い、グリアの細胞運命決定過程において重要な働きをしていることを明かにした。 10.水晶体で発現されるL-mafの機能を検証するため、これを網膜細胞に導入するとクリスタリン遺伝子が活性化され、水晶体分化因子であることが証明された。

Research Products

(74 results)

All Other

All Publications (74 results)

[Publications] Shibamoto, S. , Hayakawa,M. , Takeichi, M. , その他: "Association of p120, a tyrosine kinase substrate, with E-cadherin/catenin complexes." Journal of Cell Biology. 128. 949-957 (1995)
[Publications] Overduin, M. , Harvey, T. S. , Takeichi, M. , その他: "Three-dimensional solution structure and calcium interaction of the epithelial cadherin domain responsible for selective adhesion." Science. 267. 386-389 (1995)
[Publications] Kimura, Y. ,Matsunami, H. , Takeichi, M. , その他: "Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud of early mouse rmbryos." Developmental Biology. 169. 347-358 (1995)
[Publications] Nakagawa, S. , Takeichi, M. , その他: "Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific expression of novel cadherins." Development. 121. 1321-1332 (1995)
[Publications] Takeichi, M.: "Morphogenetic roles of classic cadherins." Ourrent Opinion in Cell Biology. 7. 619-627 (1995)
[Publications] Matsunami, H. , Takeichi, M.: "Fetal brain subdivisions defined by R-and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion." Developmental Biology. 172. 466-478 (1995)
[Publications] Uemura,T. , Oda, H. , Takeichi, M. その他: "Zygotic DE-cadherin expression is required for processes of dynamic epitheliai cell rearrangement in the Drosophila embryo." Genes & Development. (in press). (1996)
[Publications] J. A. Gross, S. Aizawa: "Control of lymphogenesis by p50csk, a regulatory protein tyrosine kinase." J. Exp. Med.181. 463-473 (1995)
[Publications] L. Hu, S. Aizawa: "p53 controls proliferation of early B lineage cells by a p21(WAF1/CIP1)-independent pathway." Biochem. Biophys. Res. Commun.206. 948-954 (1995)
[Publications] K. Sakimura, S. Aizawa: "Reduced hippocampal LTP and spatial learning in mice lacking NMDA receptor el subunit." Nature. 373. 151-155 (1995)
[Publications] D. llic, S. Aizawa: "Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells." Biochem. Biophys. Res. Commun.209. 300-309 (1995)
[Publications] K. Tobe, S. Aizawa: "Identification of a 190-kDa protein as a Novel Substrate for the Insulin Recepter Kinase Functionally Similar to Insulin Recepter Substrate-1." The Journal of Biological Chemistrey. , 270. 5698-5701 (1995)
[Publications] M. Yoshida, S. Aizawa: "A new strategy of gene-trapping in ES cells using 3′RACE." Transgenic Research. 4. 277-287 (1995)
[Publications] N. Kashiwabuch, S. Aizawa: "Impairment of coordination, purkinje cell synapse formation and cerebellar long-term depression in GluRd2 mutant mice." Cell. 81. 245-252 (1995)
[Publications] Y. Furuta, S. Aizawa: "Ovarian Teratomas in Mice Lacking the Protooncogene c-mos." Jpn. J. Cancer Res. 86. 540-545 (1995)
[Publications] K. Ikeda, S. Aizawa: "Reduced spontaneous activity of mice defective in the e4 subunit of the NMDA receptor channel." Mol. Brain Res. 33. 61-71 (1995)
[Publications] D. Ilic, S. Aizawa: "FAK-deficiency causes mesodermal defects by diminished cell mobility with enhanced focal adhesion formation." Nature. 377. 539-544 (1995)
[Publications] I. Mtsuo, S. Aizawa: "Mouse Otx2 function in the formation and patterning of rostral head." Genes & Development.9. 2646-2658 (1995)
[Publications] T. Tamura, S. Aizawa: ",An IRF-1-dependent pathway of DNA damage-induced apoptosis in mitogen-activated T lymphocytes." Nature. 376. 596-599 (1995)
[Publications] S. Kanazawa, S. Aizawa: "Integrin-stimulation decreases tyrosine phosphorylation and activity of focal adhesion kinase in thymocytes." Biophys. Biochem. Res. Commun.215. 438-445 (1995)
[Publications] Y. Furuta, S. Aizawa: "Mesodermal defect in late phase of gastrulation by a targeted mutation of focal adhesion kinase." Oncogene. 11. 1989-1995 (1995)
[Publications] S. Kuratani, S. Aizawa: "Patterning of the cranial nerves in the chick embryo is dependent on cranial mesoderm and rhombomeric metamerism." Develop. Growth Differ.37. 717-731 (1995)
[Publications] Y. Shigetani, S. Aizawa: "Overlapping origins of pharyngeal arch crest cells on the postotic hind- brain. Develop." Develop. Growth Differ.37. 733-746 (1995)
[Publications] D. Ilic, S. Aizawa: "Impairment of mobility in endodermal cells by FAK-deficiency." Exp. Cell. Res.(in press). (1995)
[Publications] S. Kanazawa, S. Aizawa: "Cooperation of p59fyn and p125FAK in Development of CD4+CD8+ Thymocytes." Blood. (in press). (1995)
[Publications] T. Sakai, S. Aizawa: "Tenascin-C inductionby the diffusible factor epidermal growth factor in stroma-epithelial interactions." J. Cell. Physiol.165. 18-29 (1995)
[Publications] M. Yasunaga,S. Aizawa: "Involvement of Fyn tyrosine kinase in progression of cytokinesis ogf B lymphocyte progenitor." J. Cell. Biol. (in press). (1995)
[Publications] Y. Enokido,S. Aizawa: "p53 involves cytosine arabinoside-induced apoptosis in cultureed cerebellar granule neurons" Neuroscience Letters. 203. 1-4 (1995)
[Publications] T.Kutsuwada, S. Aizawa: "Impairment of suckling response, trigeminal neuronal pattern formation and hippocampal LTD in NMDA receptor e2 subunit mutant mice." Neuron. (in press). (1995)
[Publications] S.Kanazawa, S. Aizawa: "Impaired development of CD4+CD8+thymocytes by csk-“knock-in" into fyn locus." Oncogene. (in press). (1995)
[Publications] M.Shimoji, S. Aizawa: "Establishment of immoratal hepatocytes from a CYP3A7-tansgenic/p53-knock out mouse." Biophys. Biochem. Res. Commun.217. 1001-1005 (1995)
[Publications] Matsumoto, M. , Okano, H. , et al.: "Ataxia and epileptic seizures in mice lacking type 1 inositol 1, 4, 5-trisphophate receptor." Nature. 379. 168-171 (1996)
[Publications] Okano, H.: "Two major mechanisms regulating cell-fate decisions in the developing nervos system." Develop. Growth. Differ.37. 619-629 (1995)
[Publications] Sawamoto, K. , Okabe, M. , Tanimura, T. , Hayashi, S. , Mikoshiba, K. , and Okano,H.: "Argos is required for optic lobe development and projection of photoreceptor axons." Developmental Dynamics. 205. 162-171 (1996)
[Publications] Okabe, M. , Sawamoto, K. , and Okano,H.: "The function of Drosophila argos gene productin the development of embryonic chordotonal organs." Developmental Biology. (in press). (1996)
[Publications] Kato, S. et al.: "Molecular cloning and expression of mouse Mg^<2+>-dependent protein phosphatase β-4(type2Cβ-4)." Arch. Biochem. Biophys.318. 387-393 (1995)
[Publications] Matsubara, N. et al.: "Murine polo like kinase 1 gene is expressed in meiotic testicular germ cells and oocytes." Mol. Reprod. Dev.41. 407-415 (1995)
[Publications] Furuichi, T. et al.: "Inhibition of testicular germ cell apoptosis and defferentiation in mice misexpressing Bcl-2 in spermatogonia." (submitted.).
[Publications] Matsubara, N. et al.: "A tyrosine kinase receptor Sky and its ligand Gas 6 are expressed in gonads and support primordial germ cell growth in culture." (submitted.).
[Publications] Tammy Greco, Shinji Takada, Andrew McMahon, & Sally Camper: "Analysis of the vestigial tail mutation demonstartes that Wnt-3a gene dosage regulates mouse axial development." Genes and Development. 印刷中 (1996)
[Publications] 高田慎治: "マウス胚発生におけるWnt遺伝子の役割" 蛋白質・核酸・酵素. 40. 2162-2174 (1995)
[Publications] Nagata, Y. et al.: "A 6-kb upstream region of the human transthyretin gene can dired developmental, tissue-specific, and quantitatively normal expression in transgenic mouse." J. Biochem.117. 169-175 (1995)
[Publications] Kitamura, D. et al.: "Molecular cloning and characterization of mouse HS1." Biochem. Biophys. Res. Commun.208. 1137-1146 (1995)
[Publications] Hashido, K. et al.: "Copy-dependent and position-independent expression of rat aldolase A gene." J. Biochem.118. 601-606 (1995)
[Publications] Hiramatsu, R. et al.: "The 3′enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin V_k -J_kjoining." Cell.83. 1113-1123 (1995)
[Publications] Yokouchi, Y.: "Coordinated expression of Abd-B subfamily genes of the HoxA cluster in the developing digestive tract of embryo." Dev. Biol.168. 76-89 (1995)
[Publications] Yokouchi, Y.: "Misexpression of Hoxa-13 induces cartilage homeotic transformation and changes cell adhesiveness in chick limb buds." Genes & Dev.9. 2509-2522 (1995)
[Publications] Morrison, A.: "Comparative analysis of chicken Hoxb-4 regulation in transgenic mice." Mech. Develop.53. 47-59 (1995)
[Publications] Kamachi, Y, Kondoh, H. et al.: "Involvement of SOX proteins in lens-specific activation of crystallin genes." EMBO, Journal. 14. 3510-3519 (1995)
[Publications] Makita, R. , Kondoh, H. , Okanoto, M.: "Transgenesis of newt with exogenous gene expression fucilitated by satellite 2 repeat." Development Growth and Differentiation. 37. 605-616 (1995)
[Publications] Kitsukaw, T. , Kondoh, H. , Fujisawa,H. et al.: "Over expression of a membrane protein, neuropilin, in chimeric. mice causes abnormalities in the cardio vasailar system, nevous system and limb." Development.121. 4309-4318 (1995)
[Publications] Shoji, h. , Kondoh, H. , Takahashi, N. et al.: "Regionali2ed expression of the Dbx fanuily homeobox gene in the embryonic CNS of the mouse." Mechanisms of Development.(印刷中). (1996)
[Publications] Ishibashi, M. , et al.: "Targeted disruption of mammalian hairy and Enhancer of split homolog-1(HES-1)leads to up-regulation of neural helix-loop-helix actors, premature neurogenesis and severe neural tobe defects." Genes & Development. 9. 3136-3148 (1995)
[Publications] Shimizu, C. , et al.: "MATH-2, a mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal, is specifically expressed in the nervous system." European Jouranal of Biochemistry. 229. 239-248 (1995)
[Publications] Akazawa, C. , et al.: "A mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal is a positive transcriptional regulator expressed in the developing nervous system." The Journal of Biological Chemistry. 270. 8730-8738 (1995)
[Publications] Takebayashi, K. , et al.: "Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5: identification of the neural precursor cell-specific promoter element." The Journal of Biological Chemistry. 270. 1342-1349 (1995)
[Publications] Tomita, K. , et al.: "Mammalian hairy and Enhancer of split homologue-1 (HES-1)regulates diffrentiation of retinal neurons and is essential for eye morphogenesis." Neuron. (in press). (1996)
[Publications] T. Takeuchi, Y. Yamazaki, Y. Katoh-Fukui, R. Tsuchiya, S. Kondo, J. Motoyama, and T. Higashinakagawa: "Gene trap capture of a novel mouse gene, jumonji, required for neural tube formation" Genes & Development. 9. 1211-1222 (1995)
[Publications] 元山純、竹内隆: "マウス遺伝子トラップ法によって得られたjumonji変異体と胚発生" 蛋白質核酸酵素. 40. 2152-2161 (1995)
[Publications] 竹内隆: "マウス遺伝子トラップ法" バイオクリニカ. (in press).
[Publications] 竹内隆: "jumonji遺伝子の神経系における発現と機能" AD&S. (in press).
[Publications] J.Motoyama, S. Tanaka, S. Kondo and T. Takeuchi: "The mouse jumonji gene is required for megakaryocyte development in the fetal liver." (submitted).
[Publications] Tokumoto M et al: "Molecular heterogeneity among primary motoneurons and within myotmes revealed by differential mRNA expression of novel Isl-1 homologs in embryonic zebrafish." Devel Biol. 171. 578-589 (1995)
[Publications] Kuo, C-H et al: "Determination of a necidin cis-acting element requied for neuron specific expression by using zebrafish." Biochem Biophys Res Comm. 211. 438-446 (1995)
[Publications] Shimoda N et al: "Mermaid, afamily of short interspersed repetitive elements widespread in vertebrates." Biochem Biophys Res Comm. (in press). (1996)
[Publications] Shimoda N et al: "Mermaid, afamily of short interspersed repetitive elements, is useful for the zebrafish genome mapping." Biochem Biophys Res Comm. (in press). (1996)
[Publications] A. Nose, M. Takeuchi and C. S. Goodman: "Molecular genetic analysis of the role of Connectin in neuromuscular recognition in Drosophila." Basic Neuroscience in Invertebrates(Japan Sci. Soc. Press). (発表予定) (1996)
[Publications] 安田國雄: "Localization, trafficking, and temperature-dependence of the Aquorea green fluorescent protein in cultured vertebrate cells" Proc. Natl. Acad. Sci. USA.92. 11899-11903 (1995)
[Publications] 安田國雄: "Involvement of a member of the maf family in lens-specific expression of the chicken αA-crystallin gene."
[Publications] 安田國雄: "A transcription factor that binds to αCE2, one of the lens-specific enhancer elements of the chicken αA-crystallin gene, is a chicken homolog of a globin gene switching factor, CP2."
[Publications] Mikoshiba, K. , Okano, H. ,Furuichi, T. , Miyawaki, A. , Furuichi, T. and Ikenaka,K.: "Molecular genetic analyses of the myelin deficiency and cerebellar ataxia in “Progress in Brain Research"" Elsevier. Eds. Yu, A. C. H. ,Eng, L. F. , Mahan, U. J. , Schulman, H. , Shooter, E. and Stadin, A., 23-41 (1995)
[Publications] Okano, H. ,Sawamoto, K. ,Okabe, M. , Yoshikawa, S. , Sakakibara, S. , Imai, T. , Kaneko, Y. , and Mikoshiba K.: "Drosophila visual system as a model to study neurobiology in general: identification of genes regulating cell fate decision in neural development.in “Basic Neuroscience in Invertebrate"" Business Center for Academic Societies Japan, Ed. H. Koike, 15-34 (1996)
[Publications] 松居靖久ほか: "生殖細胞-基礎から応用まで(岡田益吉、長濱嘉孝編)" 共立出版, 印刷中
[Publications] Okamoto H et al: "Basic Neuroscience in Invertebrate" Business Center for Academic Scieties Japan, (1996)

1995 Fiscal Year Annual Research Report

発生・形態形成の個体レベルにおける分子生物学的研究

Principal Investigator

竹市 雅敏 京都大学, 大学院・理学研究科, 教授 (00025454)

Research Products

[Publications] Shibamoto, S. , Hayakawa,M. , Takeichi, M. , その他: "Association of p120, a tyrosine kinase substrate, with E-cadherin/catenin complexes." Journal of Cell Biology. 128. 949-957 (1995)

[Publications] Overduin, M. , Harvey, T. S. , Takeichi, M. , その他: "Three-dimensional solution structure and calcium interaction of the epithelial cadherin domain responsible for selective adhesion." Science. 267. 386-389 (1995)

[Publications] Kimura, Y. ,Matsunami, H. , Takeichi, M. , その他: "Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud of early mouse rmbryos." Developmental Biology. 169. 347-358 (1995)

[Publications] Nakagawa, S. , Takeichi, M. , その他: "Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific expression of novel cadherins." Development. 121. 1321-1332 (1995)

[Publications] Takeichi, M.: "Morphogenetic roles of classic cadherins." Ourrent Opinion in Cell Biology. 7. 619-627 (1995)

[Publications] Matsunami, H. , Takeichi, M.: "Fetal brain subdivisions defined by R-and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion." Developmental Biology. 172. 466-478 (1995)

[Publications] Uemura,T. , Oda, H. , Takeichi, M. その他: "Zygotic DE-cadherin expression is required for processes of dynamic epitheliai cell rearrangement in the Drosophila embryo." Genes & Development. (in press). (1996)

[Publications] J. A. Gross, S. Aizawa: "Control of lymphogenesis by p50csk, a regulatory protein tyrosine kinase." J. Exp. Med.181. 463-473 (1995)

[Publications] L. Hu, S. Aizawa: "p53 controls proliferation of early B lineage cells by a p21(WAF1/CIP1)-independent pathway." Biochem. Biophys. Res. Commun.206. 948-954 (1995)

[Publications] K. Sakimura, S. Aizawa: "Reduced hippocampal LTP and spatial learning in mice lacking NMDA receptor el subunit." Nature. 373. 151-155 (1995)

[Publications] D. llic, S. Aizawa: "Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells." Biochem. Biophys. Res. Commun.209. 300-309 (1995)

[Publications] K. Tobe, S. Aizawa: "Identification of a 190-kDa protein as a Novel Substrate for the Insulin Recepter Kinase Functionally Similar to Insulin Recepter Substrate-1." The Journal of Biological Chemistrey. , 270. 5698-5701 (1995)

[Publications] M. Yoshida, S. Aizawa: "A new strategy of gene-trapping in ES cells using 3′RACE." Transgenic Research. 4. 277-287 (1995)

[Publications] N. Kashiwabuch, S. Aizawa: "Impairment of coordination, purkinje cell synapse formation and cerebellar long-term depression in GluRd2 mutant mice." Cell. 81. 245-252 (1995)

[Publications] Y. Furuta, S. Aizawa: "Ovarian Teratomas in Mice Lacking the Protooncogene c-mos." Jpn. J. Cancer Res. 86. 540-545 (1995)

[Publications] K. Ikeda, S. Aizawa: "Reduced spontaneous activity of mice defective in the e4 subunit of the NMDA receptor channel." Mol. Brain Res. 33. 61-71 (1995)

[Publications] D. Ilic, S. Aizawa: "FAK-deficiency causes mesodermal defects by diminished cell mobility with enhanced focal adhesion formation." Nature. 377. 539-544 (1995)

[Publications] I. Mtsuo, S. Aizawa: "Mouse Otx2 function in the formation and patterning of rostral head." Genes & Development.9. 2646-2658 (1995)

[Publications] T. Tamura, S. Aizawa: ",An IRF-1-dependent pathway of DNA damage-induced apoptosis in mitogen-activated T lymphocytes." Nature. 376. 596-599 (1995)

[Publications] S. Kanazawa, S. Aizawa: "Integrin-stimulation decreases tyrosine phosphorylation and activity of focal adhesion kinase in thymocytes." Biophys. Biochem. Res. Commun.215. 438-445 (1995)

[Publications] Y. Furuta, S. Aizawa: "Mesodermal defect in late phase of gastrulation by a targeted mutation of focal adhesion kinase." Oncogene. 11. 1989-1995 (1995)

[Publications] S. Kuratani, S. Aizawa: "Patterning of the cranial nerves in the chick embryo is dependent on cranial mesoderm and rhombomeric metamerism." Develop. Growth Differ.37. 717-731 (1995)

[Publications] Y. Shigetani, S. Aizawa: "Overlapping origins of pharyngeal arch crest cells on the postotic hind- brain. Develop." Develop. Growth Differ.37. 733-746 (1995)

[Publications] D. Ilic, S. Aizawa: "Impairment of mobility in endodermal cells by FAK-deficiency." Exp. Cell. Res.(in press). (1995)

[Publications] S. Kanazawa, S. Aizawa: "Cooperation of p59fyn and p125FAK in Development of CD4+CD8+ Thymocytes." Blood. (in press). (1995)

[Publications] T. Sakai, S. Aizawa: "Tenascin-C inductionby the diffusible factor epidermal growth factor in stroma-epithelial interactions." J. Cell. Physiol.165. 18-29 (1995)

[Publications] M. Yasunaga,S. Aizawa: "Involvement of Fyn tyrosine kinase in progression of cytokinesis ogf B lymphocyte progenitor." J. Cell. Biol. (in press). (1995)

[Publications] Y. Enokido,S. Aizawa: "p53 involves cytosine arabinoside-induced apoptosis in cultureed cerebellar granule neurons" Neuroscience Letters. 203. 1-4 (1995)

[Publications] T.Kutsuwada, S. Aizawa: "Impairment of suckling response, trigeminal neuronal pattern formation and hippocampal LTD in NMDA receptor e2 subunit mutant mice." Neuron. (in press). (1995)

[Publications] S.Kanazawa, S. Aizawa: "Impaired development of CD4+CD8+thymocytes by csk-“knock-in" into fyn locus." Oncogene. (in press). (1995)

[Publications] M.Shimoji, S. Aizawa: "Establishment of immoratal hepatocytes from a CYP3A7-tansgenic/p53-knock out mouse." Biophys. Biochem. Res. Commun.217. 1001-1005 (1995)

[Publications] Matsumoto, M. , Okano, H. , et al.: "Ataxia and epileptic seizures in mice lacking type 1 inositol 1, 4, 5-trisphophate receptor." Nature. 379. 168-171 (1996)

[Publications] Okano, H.: "Two major mechanisms regulating cell-fate decisions in the developing nervos system." Develop. Growth. Differ.37. 619-629 (1995)

[Publications] Sawamoto, K. , Okabe, M. , Tanimura, T. , Hayashi, S. , Mikoshiba, K. , and Okano,H.: "Argos is required for optic lobe development and projection of photoreceptor axons." Developmental Dynamics. 205. 162-171 (1996)

[Publications] Okabe, M. , Sawamoto, K. , and Okano,H.: "The function of Drosophila argos gene productin the development of embryonic chordotonal organs." Developmental Biology. (in press). (1996)

[Publications] Kato, S. et al.: "Molecular cloning and expression of mouse Mg^<2+>-dependent protein phosphatase β-4(type2Cβ-4)." Arch. Biochem. Biophys.318. 387-393 (1995)

[Publications] Matsubara, N. et al.: "Murine polo like kinase 1 gene is expressed in meiotic testicular germ cells and oocytes." Mol. Reprod. Dev.41. 407-415 (1995)

[Publications] Furuichi, T. et al.: "Inhibition of testicular germ cell apoptosis and defferentiation in mice misexpressing Bcl-2 in spermatogonia." (submitted.).

[Publications] Matsubara, N. et al.: "A tyrosine kinase receptor Sky and its ligand Gas 6 are expressed in gonads and support primordial germ cell growth in culture." (submitted.).

[Publications] Tammy Greco, Shinji Takada, Andrew McMahon, & Sally Camper: "Analysis of the vestigial tail mutation demonstartes that Wnt-3a gene dosage regulates mouse axial development." Genes and Development. 印刷中 (1996)

[Publications] 高田慎治: "マウス胚発生におけるWnt遺伝子の役割" 蛋白質・核酸・酵素. 40. 2162-2174 (1995)

[Publications] Nagata, Y. et al.: "A 6-kb upstream region of the human transthyretin gene can dired developmental, tissue-specific, and quantitatively normal expression in transgenic mouse." J. Biochem.117. 169-175 (1995)

[Publications] Kitamura, D. et al.: "Molecular cloning and characterization of mouse HS1." Biochem. Biophys. Res. Commun.208. 1137-1146 (1995)

[Publications] Hashido, K. et al.: "Copy-dependent and position-independent expression of rat aldolase A gene." J. Biochem.118. 601-606 (1995)

[Publications] Hiramatsu, R. et al.: "The 3′enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin V_k -J_kjoining." Cell.83. 1113-1123 (1995)

[Publications] Yokouchi, Y.: "Coordinated expression of Abd-B subfamily genes of the HoxA cluster in the developing digestive tract of embryo." Dev. Biol.168. 76-89 (1995)

[Publications] Yokouchi, Y.: "Misexpression of Hoxa-13 induces cartilage homeotic transformation and changes cell adhesiveness in chick limb buds." Genes & Dev.9. 2509-2522 (1995)

[Publications] Morrison, A.: "Comparative analysis of chicken Hoxb-4 regulation in transgenic mice." Mech. Develop.53. 47-59 (1995)

[Publications] Kamachi, Y, Kondoh, H. et al.: "Involvement of SOX proteins in lens-specific activation of crystallin genes." EMBO, Journal. 14. 3510-3519 (1995)

[Publications] Makita, R. , Kondoh, H. , Okanoto, M.: "Transgenesis of newt with exogenous gene expression fucilitated by satellite 2 repeat." Development Growth and Differentiation. 37. 605-616 (1995)

[Publications] Kitsukaw, T. , Kondoh, H. , Fujisawa,H. et al.: "Over expression of a membrane protein, neuropilin, in chimeric. mice causes abnormalities in the cardio vasailar system, nevous system and limb." Development.121. 4309-4318 (1995)

[Publications] Shoji, h. , Kondoh, H. , Takahashi, N. et al.: "Regionali2ed expression of the Dbx fanuily homeobox gene in the embryonic CNS of the mouse." Mechanisms of Development.(印刷中). (1996)

[Publications] Ishibashi, M. , et al.: "Targeted disruption of mammalian hairy and Enhancer of split homolog-1(HES-1)leads to up-regulation of neural helix-loop-helix actors, premature neurogenesis and severe neural tobe defects." Genes & Development. 9. 3136-3148 (1995)

[Publications] Shimizu, C. , et al.: "MATH-2, a mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal, is specifically expressed in the nervous system." European Jouranal of Biochemistry. 229. 239-248 (1995)

[Publications] Akazawa, C. , et al.: "A mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal is a positive transcriptional regulator expressed in the developing nervous system." The Journal of Biological Chemistry. 270. 8730-8738 (1995)

[Publications] Takebayashi, K. , et al.: "Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5: identification of the neural precursor cell-specific promoter element." The Journal of Biological Chemistry. 270. 1342-1349 (1995)

[Publications] Tomita, K. , et al.: "Mammalian hairy and Enhancer of split homologue-1 (HES-1)regulates diffrentiation of retinal neurons and is essential for eye morphogenesis." Neuron. (in press). (1996)

[Publications] T. Takeuchi, Y. Yamazaki, Y. Katoh-Fukui, R. Tsuchiya, S. Kondo, J. Motoyama, and T. Higashinakagawa: "Gene trap capture of a novel mouse gene, jumonji, required for neural tube formation" Genes & Development. 9. 1211-1222 (1995)

[Publications] 元山純、竹内隆: "マウス遺伝子トラップ法によって得られたjumonji変異体と胚発生" 蛋白質 核酸 酵素. 40. 2152-2161 (1995)

[Publications] 竹内隆: "マウス遺伝子トラップ法" バイオクリニカ. (in press).

[Publications] 竹内隆: "jumonji遺伝子の神経系における発現と機能" AD&S. (in press).

[Publications] J.Motoyama, S. Tanaka, S. Kondo and T. Takeuchi: "The mouse jumonji gene is required for megakaryocyte development in the fetal liver." (submitted).

[Publications] Tokumoto M et al: "Molecular heterogeneity among primary motoneurons and within myotmes revealed by differential mRNA expression of novel Isl-1 homologs in embryonic zebrafish." Devel Biol. 171. 578-589 (1995)

[Publications] Kuo, C-H et al: "Determination of a necidin cis-acting element requied for neuron specific expression by using zebrafish." Biochem Biophys Res Comm. 211. 438-446 (1995)

[Publications] Shimoda N et al: "Mermaid, afamily of short interspersed repetitive elements widespread in vertebrates." Biochem Biophys Res Comm. (in press). (1996)

[Publications] Shimoda N et al: "Mermaid, afamily of short interspersed repetitive elements, is useful for the zebrafish genome mapping." Biochem Biophys Res Comm. (in press). (1996)

[Publications] A. Nose, M. Takeuchi and C. S. Goodman: "Molecular genetic analysis of the role of Connectin in neuromuscular recognition in Drosophila." Basic Neuroscience in Invertebrates(Japan Sci. Soc. Press). (発表予定) (1996)

[Publications] 安田 國雄: "Localization, trafficking, and temperature-dependence of the Aquorea green fluorescent protein in cultured vertebrate cells" Proc. Natl. Acad. Sci. USA.92. 11899-11903 (1995)

[Publications] 安田 國雄: "Involvement of a member of the maf family in lens-specific expression of the chicken αA-crystallin gene."

[Publications] 安田 國雄: "A transcription factor that binds to αCE2, one of the lens-specific enhancer elements of the chicken αA-crystallin gene, is a chicken homolog of a globin gene switching factor, CP2."

[Publications] 松居靖久 ほか: "生殖細胞-基礎から応用まで(岡田益吉、長濱嘉孝編)" 共立出版, 印刷中

[Publications] Okamoto H et al: "Basic Neuroscience in Invertebrate" Business Center for Academic Scieties Japan, (1996)

竹市雅敏京都大学, 大学院・理学研究科, 教授 (00025454)

[Publications] 元山純、竹内隆: "マウス遺伝子トラップ法によって得られたjumonji変異体と胚発生" 蛋白質核酸酵素. 40. 2152-2161 (1995)

[Publications] 安田國雄: "Localization, trafficking, and temperature-dependence of the Aquorea green fluorescent protein in cultured vertebrate cells" Proc. Natl. Acad. Sci. USA.92. 11899-11903 (1995)

[Publications] 安田國雄: "Involvement of a member of the maf family in lens-specific expression of the chicken αA-crystallin gene."

[Publications] 安田國雄: "A transcription factor that binds to αCE2, one of the lens-specific enhancer elements of the chicken αA-crystallin gene, is a chicken homolog of a globin gene switching factor, CP2."

[Publications] 松居靖久ほか: "生殖細胞-基礎から応用まで(岡田益吉、長濱嘉孝編)" 共立出版, 印刷中